C57BL/6-Tg(Cnr1-EGFP)1Mirn/J

Stock number: 028597
Product name: Cnr1-eGFP
Research areas: Neurobiology Research, Research Tools

Description

The Cnr1-eGFP line 1 BAC transgenic mouse line has the endogenous mouse cannabinoid receptor 1 promoter/enhancer regions directing fluorescent reporter expression. These mice may be useful in studying CNR1+ GABAergic interneurons in behavioral and molecular processes, as well as cell-type specific GABAergic disturbances in manifestations of schizophrenia.

Detailed description

Cnr1-eGFP line 1 BAC transgenic mice have eGFP fluorescent protein expression directed by the mouse cannabinoid receptor 1 promoter/enhancer regions on the BAC. The morphology and distribution of the labeled interneurons across the different brain structures show that the transgene is specifically expressed in the cell sub-populations that normally express the Cnr1 gene; including GABAergic interneuron basket cells (smaller subpopulation of CCK+ basket cells). Strong eGFP fluorescence is reported specifically in CNR1+ cells across all tested brain regions (cortex, hippocampus, substantia nigra, amygdala and cerebellum), with strong labeling of neuronal somata and synaptic terminals.
Hemizygous mice are viable and fertile. To date (December 2015), it has not been attempted to make this strain homozygous.

Development

The Cnr1-eGFP line 1 BAC transgenic mouse line (also called Cnr1-eGFP-lox line 1) was created by Dr. Karoly Mirnics (Vanderbilt University) by breeding Cnr1-eGFP-miGAD67 line 1 BAC transgenic mice (see Stock No. 028593) to mice expressing Cre recombinase in germline (C57BL/6-Tg(Zp3-cre)93Knw/J; Stock No. 003651). Offspring with germline deletion of the miRNA:Gad1-containing β-globin minigene were identified (Cnr1-eGFP), and then bred to C57BL/6NHsd to remove the Zp3-Cre transgene. The Cnr1-eGFP colony was backcrossed to C57BL/6NHsd for a total of five generations, and then sent to The Jackson Laboratory in 2012. Upon arrival, sperm was cryopreserved. If generating a live colony, an aliquot of frozen sperm may be used to fertilize C57BL/6NJ oocytes (Stock No. 005304).

Allele

Symbol: Tg(Cnr1-EGFP)1Mirn
Name: transgene insertion 1, Karoly Mirnics
MGI accession ID: MGI:5703927
Generation method: Transgenic
Attributes: Reporter
Synonyms: Cnr1-eGFP line 1
Marker symbol: Tg(Cnr1-EGFP)1Mirn
Marker name: transgene insertion 1, Karoly Mirnics
Chromosome: UN
Strain of origin: C57BL/6NTac
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP fluorescence is reported in CNR1+ cells across all tested brain regions (cortex, hippocampus, substantia nigra, amygdala and cerebellum), with strong labeling of neuronal somata and synaptic terminals.
Molecular note: Cre-mediated recombination removed the target I sequence between exon IX and exon X of Tg(Cnr1-EGFP/RNAi:Gad1)1Mirn resulting in fluorescent reporter expression in the cell sub-populations that normally express the Cnr1 gene; including GABAergic interneuron basket cells (smaller subpopulation of CCK+ basket cells).