B6;129S4-Gt(ROSA)26Sor^{tm5(CAG-flpo)Zjh}/J

Stock number: 028584
Product name: LSL-Flpo
Research areas: Research Tools

Description

These LSL-Flpo mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette and a Flp Recombinase sequence. This strain may be useful for the conversion from Cre-mediated to Flp-mediated excision.
Of note, a similar but non-inducible (constitutive) FLPo-expressing strain is available on a congenic C57BL/6 background - Stock No. 012930 (B6 ROSA26Flpo).

Detailed description

LSL-Flpo mice homozygous for this Rosa-CAG-LSL-Flp conditional allele are viable and fertile. A loxP-flanked STOP cassette prevent transcription of the downstream Flp Recombinase sequence. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Flp Recombinase expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the loxP-flanked STOP cassette deleted in the cre-expressing tissue(s), resulting in Flp recombinase expression.

Development

The LSL-Flpo targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a Flpo Recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), and a Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation of (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were selected and chimeric males were bred to C57BL/6J females and offspring were subsequently bred together to create a homozygous colony of LSL-Flpo mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Gt(ROSA)26Sor^{tm5(CAG-flpo)Zjh}
Name: targeted mutation 5, Z Josh Huan
MGI accession ID: MGI:5700398
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Recombinase-expressing
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Expressed genes: FLP,FLP recombinase,Saccharomyces cerevisiae
Site of expression: Flp recombinase is expressed in cre-expressing tissues after breeding with a strain carrying cre recombinase.
Molecular note: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a flpo recombinase gene (a codon-optimized flpe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), and a Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.