These HG-dual mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette and a frt-flanked STOP cassette preventing transcription of a CAG promoter-driven enhanced green fluorescent protein (EGFP). EGFP is only expressed following Cre-mediated and Flp-mediated recombination. This strain may be useful as an intersectional reporter for cre and FLP expression.
Z. Josh Huang, Cold Spring Harbor Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
HG-dual intersectional reporter mice homozygous for this Rosa-CAG-LSL-FSF-H2B-EGFP conditional allele are viable and fertile. A loxP-flanked STOP cassette and a frt-flanked STOP cassette prevent transcription of the downstream H2B-EGFP fusion protein. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EGFP expression is determined by which tissue(s) express Cre recombinase and, subsequently express Flp recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the loxP-flanked STOP cassette deleted in the cre-expressing tissue(s). After cre excision, expression of EGFP is restricted by the frt-flanked STOP cassette. After removal of the two STOP cassettes by Cre- and FLP-recombination, EGFP fluorescence is expected in cells/tissues where the expression patterns of the individual promoters driving cre and Flp overlap. The donating investigator also reports that the order in which the loxP-flanked STOP and frt-flanked STOP cassettes are removed is arbitrary.
The HG-dual targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a frt-flanked STOP cassette, a H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by an enhanced green fluorescent protein sequence), and a Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation of (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were selected and chimeric males were bred to C57BL/6J females and offspring were subsequently bred together to create a homozygous colony of HG-dual mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | H2B-eGFP, Histone H2B-enhanced Green Fluorescent Protein, |
---|---|
Site of Expression | After Cre- and FLP-recombination, EGFP fluorescence is expected in cells/tissues where the expression patterns of the individual promoters driving cre and Flp overlap |
Allele Name | targeted mutation 2, Z Josh Huang |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | HG-dual |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | H2B-eGFP, Histone H2B-enhanced Green Fluorescent Protein, |
Site of Expression | After Cre- and FLP-recombination, EGFP fluorescence is expected in cells/tissues where the expression patterns of the individual promoters driving cre and Flp overlap |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 6 |
Molecular Note | The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a FRT-flanked STOP cassette, an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by an enhanced green fluorescent protein sequence), and a Neo-polyA cassette. The entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. |
When maintaining a live colony, homozygous mice may be bred together.
When using the HG dual mouse strain in a publication, please cite the originating article(s) and include JAX stock #028581 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm2(CAG-HIST1H2BB/EGFP)Zjh> |
Frozen Mouse Embryo | B6;129S4-Gt(ROSA)26Sor<tm2(CAG-HIST1H2BB/EGFP)Zjh>/J Frozen | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Gt(ROSA)26Sor<tm2(CAG-HIST1H2BB/EGFP)Zjh>/J Frozen | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Gt(ROSA)26Sor<tm2(CAG-HIST1H2BB/EGFP)Zjh>/J Frozen | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Gt(ROSA)26Sor<tm2(CAG-HIST1H2BB/EGFP)Zjh>/J Frozen | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.