PV-2A-CreER knock-in mice express the CreERT2 fusion protein in parvalbumin positive neurons of the neocortex and cerebellum, as directed by the endogenous Pvalb promoter/enhancer elements.
Z. Josh Huang, Cold Spring Harbor Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible) | Pvalb | parvalbumin |
PV-2A-CreER knock-in mice express a CreERT2 fusion protein (creERT2 fusion protein) from the Pvalb promoter/enhancer elements. Homozygous mice are viable and fertile. Cre-ERT2 fusion gene activity is inducible; observed following tamoxifen administration. As such, when Pv-CreERT2 mice are bred with mice containing loxP-flanked sequence(s), tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequence(s) in parvalbumin positive neurons in neocortex and cerebellum of the offspring.
When PV-2A-CreER mice are bred to RGBow reporter mice (Stock No. 028583), tamoxifen-inducible Cre-mediated recombination results in fluorescently labeled neocortical SST and PV interneurons in three colors randomly.
PV-2A-CreER mice have CreERT2 fusion protein inserted downstream of the stop codon of the targeted gene, allowing this strain to express endogenous Pvalb as well as CreERT2. The donating investigator reports that this strain is superior to Pv-CreERT2 (Stock No. 010777) in which CreERT2 is inserted into the initiation codon of the Pvalb gene, creating a knockin/knockout allele.
A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), followed by a frt-flanked neo cassette, upstream of the stop codon of the parvalbumin (Pvalb) gene. This construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred together to create a homozygous colony of PV-2A-CreER mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | Driven by Pvalb promoter/enhancer elements following induction by tamoxifen, cre/ERT2 fusion protein is expressed in parvalbumin positive neurons in neocortex and cerebellum of the offspring. |
Allele Name | targeted mutation 2, Z Josh Huang |
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Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | PV-2A-CreEr; PV-CreER |
Gene Symbol and Name | Pvalb, parvalbumin |
Gene Synonym(s) | |
Promoter | Pvalb, parvalbumin, mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Driven by Pvalb promoter/enhancer elements following induction by tamoxifen, cre/ERT2 fusion protein is expressed in parvalbumin positive neurons in neocortex and cerebellum of the offspring. |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 15 |
Molecular Note | A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), followed by a frt-flanked neo cassette, upstream of the stop codon. |
When maintaining a live colony, homozygous mice may be bred together.
When using the PV-2A-CreER mouse strain in a publication, please cite the originating article(s) and include JAX stock #028580 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pvalb<tm2.1(cre/ERT2)Zjh> |
Frozen Mouse Embryo | B6;129S4-Pvalb<tm2(cre/ERT2)Zjh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Pvalb<tm2(cre/ERT2)Zjh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Pvalb<tm2(cre/ERT2)Zjh>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Pvalb<tm2(cre/ERT2)Zjh>/J Frozen Embryo | $3373.50 |
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