B6;129S4-Pvalb^{tm2(cre/ERT2)Zjh}/J

Stock number: 028580
Product name: PV-2A-CreER
Research areas: Neurobiology Research, Research Tools

Description

PV-2A-CreER knock-in mice express the CreERT2 fusion protein in parvalbumin positive neurons of the neocortex and cerebellum, as directed by the endogenous Pvalb promoter/enhancer elements.

Detailed description

PV-2A-CreER knock-in mice express a CreER^T2 fusion protein (creERT2 fusion protein) from the Pvalb promoter/enhancer elements. Homozygous mice are viable and fertile. Cre-ERT2 fusion gene activity is inducible; observed following tamoxifen administration. As such, when Pv-CreERT2 mice are bred with mice containing loxP-flanked sequence(s), tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequence(s) in parvalbumin positive neurons in neocortex and cerebellum of the offspring.

When PV-2A-CreER mice are bred to RGBow reporter mice (Stock No. 028583), tamoxifen-inducible Cre-mediated recombination results in fluorescently labeled neocortical SST and PV interneurons in three colors randomly.

PV-2A-CreER mice have CreER^T2 fusion protein inserted downstream of the stop codon of the targeted gene, allowing this strain to express endogenous Pvalb as well as CreER^T2. The donating investigator reports that this strain is superior to Pv-CreERT2 (Stock No. 010777) in which CreER^T2 is inserted into the initiation codon of the Pvalb gene, creating a knockin/knockout allele.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a CreER^T2 fusion gene (Cre-ER^T2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), followed by a frt-flanked neo cassette, upstream of the stop codon of the parvalbumin (Pvalb) gene. This construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred together to create a homozygous colony of PV-2A-CreER mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Pvalb^{tm2(cre/ERT2)Zjh}
Name: targeted mutation 2, Z Josh Huang
MGI accession ID: MGI:5700403
Generation method: Targeted
Attributes: Recombinase-expressing; Inducible
Marker symbol: Pvalb
Marker name: parvalbumin
Chromosome: 15
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Driven by Pvalb promoter/enhancer elements following induction by tamoxifen, cre/ERT2 fusion protein is expressed in parvalbumin positive neurons in neocortex and cerebellum of the offspring.
Molecular note: A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), followed by a frt-flanked neo cassette, upstream of the stop codon.