B6;SJL-Tg(Cyp17a1-icre)AJako/J

Stock number: 028547
Product name: Cyp17iCre
Research areas: Reproductive Biology Research, Research Tools

Description

These Cyp17iCre transgenic mice have the mouse Cyp17a1 promoter driving codon optimized iCre recombinase expression in the ovarian theca/interstitial cells and Leydig cells in the testis.

Detailed description

The Cyp17a1 gene encodes a cytochrome P450 enzyme that is involved in fetal and pubescent sexual development. These transgenic mice express iCre recombinase under the control of the mouse cytochrome P450, family 17, subfamily a, polypeptide 1, gene promoter. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the Cre expressing tissues. Cre recombinase activity is detected in ovarian theca and interstitial cells, testis Leydig cells, pituitary gland intermediate lobe and liver. Weaker Cre recombinase activity is observed in the oviduct and uterus, and in gonadotropin-primed ovarian granulosa cells, as well as kidney, adrenal gland, spleen, lung and brain. At embryonic day 16.5, Cre recombinase is expressed in the testis. Mice that are homozygous for the transgene are viable and fertile.

Development

A transgenic construct containing codon optimized (icre) under the control of the mouse Cyp17a1, cytochrome P450, family 17, subfamily a, polypeptide 1, gene promoter, was injected into fertilized B6;SJL mouse eggs. Founder line A was subsequently established. The mice were maintained on the B6;SJL background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(Cyp17a1-icre)AJako
Name: transgene insertion A, CheMyong Ko
MGI accession ID: MGI:3807212
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Cyp17a1-icre)AJako
Marker name: transgene insertion A, CheMyong Ko
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is expressed in the theca/interstitial cells of the ovary and Leydig cells of the testes.
Molecular note: The promoter was used to drive expression of a codon improved cre in the theca/interstitial cells of the ovary and Leydig cells of the testes. Three lines (lines A, B and C) were created. While all lines exhibit gonadal expression, line B and C exhibit extra-gonadal expression.