B6(Cg)-Mir203^tm1.1Yir/J

Stock number: 028362
Product name: miR-203^fl/fl
Research areas: Cancer Research, Cell Biology Research, Dermatology Research, Research Tools

Description

These mice carry a floxed allele of the mouse Mir203 locus. Cre-mediated excision of the loxP-flanked region produces a knockout allele useful in studies of skin cancer.

Detailed description

Messenger RNA (mRNA) and microRNA sequencing (miR-Seq) screens of the transcriptome has revealed that Mir203 (microRNA 203) expression is downregulated in keratinocytes in response to the G12V mutation of Hras (a potent driver of tumorigenesis in skin that stimulates clonal expansion in papillomas).

These mice carry a floxed allele of the mouse Mir203 locus which enables the generation of cre-mediated conditional knockouts.

When crossed with EIIa-Cre mice, mice exhibit mild epidermal hyperplasia during skin development and have increased skin tumor burden in the DMBA/TPA skin chemical carcinogenesis model. Loss of Mir203 expression promotes selection and expansion of tumor-initiating cells. Mir203 acts to limit the proliferation of skin cells when they are rapidly dividing.

Development

LoxP sites were introduced on either side of pre-Mir203 sequences and an FRT-flanked neomycin cassette via homologous recombination in B6(Cg)-Tyr^c-2J/J- derived Cy2.4 ES cells. Resultant mice were bred with a C57BL/6J-background ACTB-Flpe strain to excise the neomycin cassette. The albino Tyr^c-2J allele was bred out of the line. This strain was maintained on a C57BL/6J background by the donating lab.

Allele

Symbol: Mir203^tm1.1Yir
Name: targeted mutation 1.1, Rui Yi
MGI accession ID: MGI:5698058
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Mir203
Marker name: microRNA 203
Chromosome: 12
Strain of origin: B6(Cg)-Tyr^c-2J
Molecular note: The targeting constructs contains a loxP site upstream of the Mir203 hairpin loop and a FRT-flanked neomycin cassette followed by a loxP site downstream of the hairpin loop. Flp-mediated recombination removed the FRT-flanked neo cassette.