C57BL/6NJ-Gpr15^em2J/J

Stock number: 028324

Description

This is a conditional by inversion (COIN) allele with a lox71/lox66 flanked EGFP inserted in inverse orientation into the antisense strand of exon 1. This is functionally a wild-type allele, but cre-mediated recombination will yield a null allele detectable by expression of enhanced green fluorescent protein.

Detailed description

No abnormal phenotype has been detected in this un-recombined COIN allele. This allele is valuable for cre-induced selective inactivation of this gene.

Development

This allele was generated by inserting a conditional by inversion (COIN) cassette containing a rabbit beta-globin polyA site, EGFP and inverted lox site variants (lox71/lox66). A plasmid was synthesized containing 1 kb homology arms and two silent mutations (c.24C>T and c.27G>T). Double-stranded plasmid DNA, Cas9 mRNA, and sgRNA were introduced into C57BL/6NJ zygotes via pronuclear injection. The founder was backcrossed to C57BL/6NJ and sperm was cryopreserved from heterozygous males at generation N3.

Allele

Symbol: Gpr15^em2J
Name: endonuclease-mediated mutation 2, Jackson
MGI accession ID: MGI:6506232
Generation method: Endonuclease-mediated
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Gpr15
Marker name: G protein-coupled receptor 15
Chromosome: 16
Strain of origin: C57BL/6NJ
Molecular note: This allele was generated by inserting a conditional by inversion (COIN) cassette containing a rabbit beta-globin polyA site, EGFP and inverted lox site variants (lox71/lox66) in inverse orientation into the antisense strand exon1. This is functionally a wild-type allele, but cre-mediated recombination will yield a null allele detectable by expression of enhanced green fluorescent protein.