B6.Cg-Tg(tetO-DRD3)3-10Kndl/J

Stock number: 028295
Product name: tetO-D3R
Strain synonyms: tetO-hD3-10
Research areas: Neurobiology Research, Research Tools

Description

Human DRD3 is conditionally expressed under the regulatory control of the tetO promoter in these tetO-D23 mice. When combined with a tTA allele, overexpression of DRD3 can be directed to tissues/cells of interest. Administration of doxycycline blocks expression of DRD3.

Detailed description

TetO transgenic mice capable of conditional overexpression of human D3R (DRD3, dopamine receptor D3) were generated to investigate the potential involvement of the gene in schizophrenia.

When crossed with Camk2a-tTA mice (e.g. Stock No. 003010), expression of DRD3 is restricted to the striatum in a reversible manner. Compound mutant mice overexpressing D3R show no abnormalities in basic behavioral functions or cognitive tests but do display a deficit in incentive motivation. This suggests that changes in D3Rs may be involved in the negative symptoms of schizophrenia. Overexpression of DRD3 may be switched off through the administration of doxycycline.

Development

The tetO promoter, consisting of seven operator sequences of the E. coli Tn10 teteracycline resistance operon and a Cytomegalovirus (CMV) minimal promoter, drives expression on the human DRD3 (D3R) gene and hGHpA (2.1 kb of the human growth hormone gene harboring the hGH polyadenlyation signal). The transgenic vector was injected into B6CBAF2 blastocysts. Resultant mice were backcrossed to C57BL/6J for more than 15 generations by the donating lab.

Allele

Symbol: Tg(tetO-DRD3)3-10Kndl
Name: transgene insertion 3-10, Eric R Kandel
MGI accession ID: MGI:5693547
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-DRD3)3-10Kndl
Marker name: transgene insertion 3-10, Eric R Kandel
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: DRD3,dopamine receptor D3,human
Molecular note: The tetO promoter consisting of seven operator sequences of the E. coli Tn10 tetracycline resistance operon and a Cytomegalovirus (CMV) minimal promoter, drives expression of the human DRD3 (D3R) gene and hGHpA (2.1 kb of the human growth hormone gene harboring the hGH polyadenylation signal).