Human DRD3 is conditionally expressed under the regulatory control of the tetO promoter in these tetO-D23 mice. When combined with a tTA allele, overexpression of DRD3 can be directed to tissues/cells of interest. Administration of doxycycline blocks expression of DRD3.
Eric R Kandel, Columbia University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
TetO transgenic mice capable of conditional overexpression of human D3R (DRD3, dopamine receptor D3) were generated to investigate the potential involvement of the gene in schizophrenia.
When crossed with Camk2a-tTA mice (e.g. Stock No. 003010), expression of DRD3 is restricted to the striatum in a reversible manner. Compound mutant mice overexpressing D3R show no abnormalities in basic behavioral functions or cognitive tests but do display a deficit in incentive motivation. This suggests that changes in D3Rs may be involved in the negative symptoms of schizophrenia. Overexpression of DRD3 may be switched off through the administration of doxycycline.
The tetO promoter, consisting of seven operator sequences of the E. coli Tn10 teteracycline resistance operon and a Cytomegalovirus (CMV) minimal promoter, drives expression on the human DRD3 (D3R) gene and hGHpA (2.1 kb of the human growth hormone gene harboring the hGH polyadenlyation signal). The transgenic vector was injected into B6CBAF2 blastocysts. Resultant mice were backcrossed to C57BL/6J for more than 15 generations by the donating lab.
Expressed Gene | DRD3, dopamine receptor D3, human |
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Site of Expression |
Allele Name | transgene insertion 3-10, Eric R Kandel |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | tetO-D3R |
Gene Symbol and Name | Tg(tetO-DRD3)3-10Kndl, transgene insertion 3-10, Eric R Kandel |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | DRD3, dopamine receptor D3, human |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | UN |
Molecular Note | The tetO promoter consisting of seven operator sequences of the E. coli Tn10 tetracycline resistance operon and a Cytomegalovirus (CMV) minimal promoter, drives expression of the human DRD3 (D3R) gene and hGHpA (2.1 kb of the human growth hormone gene harboring the hGH polyadenylation signal). |
Hemizygotes are viable and fertile.
When using the tetO-D3R mouse strain in a publication, please cite the originating article(s) and include JAX stock #028295 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier or Tg(tetO-DRD3)3-10Kndl |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-DRD3)3-10Kndl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-DRD3)3-10Kndl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-DRD3)3-10Kndl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-DRD3)3-10Kndl/J Frozen Embryo | $3373.50 |
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