These transgenic mice express green fluorescent calcium indicator GCaMP6f in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The transgene contains a mouse <i>Thy1</i> promoter, GCaMP6f, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics (<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=23868258">PMID 23868258</a> and <a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=25250714">PMID 25250714</a>). The mice can be used for imaging-based monitoring of neuronal activity in individual neurons in the brain and elsewhere. 
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Functional imaging experiments require 50-100mW average laser power (940 nm) as compared to alternative 10-25 mW in GCaMP viral transduction protocols. Stable expression levels of the GP5.1 line is reported to be strong in the hippocampus.  Moderate signal has been reported in the olfactory bulb, M1, piriform area, amygdala, S1, and V1. Weak signal is reported in the thalamus, hypothalamus, cerebellum, midbrain, and pons.  No signal is reported in the medulla.
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These mice were made by the Genetically-Encoded Neuronal Indicator and Effector (<a href="http://www.janelia.org/team-project/genie">GENIE</a>) Project at the HHMI's Janelia Farm Research Campus.


These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector (<a href="http://www.janelia.org/team-project/genie">GENIE</a>) Project, express the green fluorescent calcium indicator, GCaMP6f, in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics.

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