The trithorax group transcription activator encoded by the ash1 (absent, small, or homeotic)-like (Drosophila), Ash1l, gene, functions as a histone methyltransferase that is important for the regulation of expression of Hox genes. These mice carry a hypomorphic &beta;-geo gene trap allele of the Ash1l gene.
Homozygotes have a partially penetrant perinatal lethal phenotype. Homozygous females are infertile because of decidualization and/or implantation failure, due to fewer endometrial uterine glands. Homozygous males exhibit reduced fertility due to structural abnormalities of the epididymis, resulting in a decrease in viable sperm exiting the vas deferens. 
Significantly reduced mRNA levels are detected by real-time quantitative RT-PCR of epididymides, ductus deferens and uterus tissue, and by in situ hybridization in the uterus. Immunohistochemical analysis of uterus tissue from 3 and 4 week old homozygous females and epididymis from homozygous males also shows diminished Ash1l gene product expression (protein). Homozygotes are smaller than wildtype littermates and are born with closed eyelids. 
Adult homozygotes lack meibomian glands in the eyelids, which are necessary for eye lubrication, resulting in chronic blepharitis. Abdominal fat is reduced and thoracic verterbral abnormalities (extra rib, abnormal rib attachment to sternum) are observed in mutants.

This gene trap mutation of the Ash1l gene is a hypomorphic allele. These mice may be useful for studying the reproduction (fertility), and early embryonic growth and development.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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STOCK Ash1l<Gt(AL0395)Wtsi>/SacJ Frozen Embryo