A transgenic construct designed by Dr. Andrew Arnold (University of Connecticut School of Medicine, Farmington, Connecticut) containing a 16kb genomic fragment of the human CCND1 gene (all exons and introns, 1.1kb 5'-flanking region, and 1kb 3'-flanking region) under the control of the human PTH gene promoter (approximately 5 kb of upstream, 5'-regulatory region, and the first 66 bp of untranslated/non-coding exon 1), was injected into the pronuclei of fertilized FVB/N mouse eggs. Founder line PC2, which has an estimated 11 copies of the transgene, was subsequently established. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).
