B6.129P2(Cg)-Pax8^{tm1.1(cre)Mbu}/J

Stock number: 028196
Product name: Pax8^cre
Research areas: Developmental Biology Research, Research Tools

Description

This Pax8^cre knockin strain may be useful for generating conditional mutations for studying kidney and thyroid development.

Detailed description

The PAX8 transcription factor is important in kidney and thyroid follicular cell development. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes exhibit a postnatal lethal phenotype. Cre activity is similar to the wildtype gene expression pattern and is detected in the inner ear by E16.5; the brain beginning at E9.5; the mesonephros starting at approximately E9.5; geniculate ganglions at E10.5; thyroid gland, vestibulocochlear nerve, epididymis, and adrenal gland at E15.5. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Development

A targeting vector containing the cre recombinase gene, a FRT site flanked NEO cassette and SV40 polyadenylation site was used to disrupt exon 3 of the targeted gene. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were bred to C57BL/6 females to generate Pax8^neo/+ mice. The mice were backcrossed to C3H/He for 4 generations. The mice were then crossed to the FLPe recombinase-expressing deleter strain on the C57BL/6 genetic background to remove the FRT flanked NEO cassette, producing the Pax8^cre. The mice were then backcrossed to C57BL/6J for more than 20 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Pax8^{tm1.1(cre)Mbu}
Name: targeted mutation 1.1, Meinrad Busslinger
MGI accession ID: MGI:3050216
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Pax8
Marker name: paired box 8
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase activity is detected in known Pax8 expression domains including the developing thyroid gland, inner ear, kidney, and mid-hindbrain region.
Molecular note: Crossing Pax8^tm1(cre)Mbu with mice expressing FLP recombinase in the germline created this allele in which the frt-flanked neo downstream of the inserted Cre was excised, allowing Cre to be expressed. Expression of cre recombinase was detected in the known Pax8 expression domains; in addition, expression was detected in the adrenal gland, and in the facial, vestibulocochlear and cuneate nerves.