Csf1rdex5 KO mice lack exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene and may be useful in studying the role of CSF1R in development.
Jeffrey W Pollard, Albert Einstein College of Medicine
Csf1rdex5 KO mice lack exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene, abolishing gene expression. Csf1r encodes Cd115 which acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Expressed in macrophages, expression is also seen in oocytes, osteoclasts, trophoblasts, CNS microglia, and some myoblasts. Heterozygous Csf1rdex5 mice are viable and fertile, while, homozygous mice die by 3 weeks of age. They also have smaller body sizes compared to wildtype mice. Mice have osteoporosis and lack teeth.
A targeting vector was designed to insert a single loxP site upstream of exon 5, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene. The construct was electroporated into STOCK (129/Sv and C57BL/6J and SJL)-derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. These Csf1rfl/fl mice were bred to C57BL/6J mice for at least 15 generations and were sent to The Jackson Laboratory Repository as Stock No. 021212. Subsequently, the donating investigator bred some of these mice to B6.FVB-Tg(Zp3-cre)3Mrt/J (Stock No. 003394) to delete the neo cassette and exon 5. Upon arrival of these Csf1rdex5 mice at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Jeffrey W Pollard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Csf1rdex5; Csf1rtmdex5jwp|
|Gene Symbol and Name||Csf1r, colony stimulating factor 1 receptor|
|Strain of Origin||STOCK 129/Sv and C57BL/6J and SJL|
|Molecular Note||A targeting vector was designed to insert a single loxP site upstream of exon 5, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 5. Cre-mediated recombination removed the neo cassette and exon 5.|
When maintaining a live colony heterozygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Homozygous mice die by 3 weeks of age.
When using the Csf1rdex5 mouse strain in a publication, please cite the originating article(s) and include JAX stock #028064 in your Materials and Methods section.