B6.Cg-Csf1r^tm1.1Jwp/J

Stock number: 028064
Product name: Csf1r^dex5
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

Csf1r^dex5 KO mice lack exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene and may be useful in studying the role of CSF1R in development.

Detailed description

Csf1r^dex5 KO mice lack exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene, abolishing gene expression. Csf1r encodes Cd115 which acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Expressed in macrophages, expression is also seen in oocytes, osteoclasts, trophoblasts, CNS microglia, and some myoblasts. Heterozygous Csf1r^dex5 mice are viable and fertile, while, homozygous mice die by 3 weeks of age. They also have smaller body sizes compared to wildtype mice. Mice have osteoporosis and lack teeth.

Development

A targeting vector was designed to insert a single loxP site upstream of exon 5, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene. The construct was electroporated into STOCK (129/Sv and C57BL/6J and SJL)-derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. These Csf1r^fl/fl mice were bred to C57BL/6J mice for at least 15 generations and were sent to The Jackson Laboratory Repository as Stock No. 021212. Subsequently, the donating investigator bred some of these mice to B6.FVB-Tg(Zp3-cre)3Mrt/J (Stock No. 003394) to delete the neo cassette and exon 5. Upon arrival of these Csf1r^dex5 mice at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Csf1r^tm1.1Jwp
Name: targeted mutation 1.1, Jeffrey W Pollard
MGI accession ID: MGI:5689434
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Csf1r
Marker name: colony stimulating factor 1 receptor
Chromosome: 18
Strain of origin: STOCK 129/Sv and C57BL/6J and SJL
Molecular note: A targeting vector was designed to insert a single loxP site upstream of exon 5, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 5. Cre-mediated recombination removed the neo cassette and exon 5.