Overview

Also Known As: Human COMT Val, Val/Val

Human COMT Val mice have the human COMT gene replacing the entire coding region of the endogenous gene. They may be useful when studying schizophrenia and other psychiatric disorders.

Donating Investigator

Xianjin Zhou, University of California San Diego

Genetic overview

Genetic Background	Generation

Comt^{tm1(COMT)Xzho}

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout, Inserted expressed sequence)	Comt	catechol-O-methyltransferase

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Research Applications

Neurobiology Research

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Base Price

Starting at:

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Details

Detailed Description

Human COMT Val mice contain human catechol-O-methyltransferase (Comt) gene replacing the endogenous coding region. Membrane bound COMT is highly expressed in both prefrontal cortex and hippocampus and is a key enzyme in catecholamine clearance from the synapse. COMT helps maintain appropriate levels of neurotransmitters, such as such as dopamine and norepinephrine, in prefrontal cortex. Mutations and increased frequency of this gene are associated with schizophrenia, anxiety, mood disorders, obsessive-compulsive disorder, and attention deficit disorder. Homozygous Human COMT Val mice are viable and fertile. The donating investigator reports a lack of phenotype on this 129 genetic background (July 2018). We may modify the strain description if necessary as any results become available.

Of note, this allele is also available on a C57BL/6J congenic background as Stock No. 032382.

Companion strains, containing containing a Val158Met mutation, commonly associated with the development of schizophrenia and other psychiatric disorders, are available on a congenic B6J background (Stock No. 032383) and a 129 coisogenic background (Stock No. 027993). The congenic B6J Val158Met strain shows behavioral phenotypes consistent with those seen in patients with schizophrenia and other psychiatric disorders.

Development

The Human COMT Val targeting construct was designed to replace the entire endogenous catechol-O-methyltransferase (Comt) coding region with human Comt sequence followed by a neomycin (neo) resistance cassette. This targeting construct was electroporated into 129Sv-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. Human COMT Val mice were maintained on a 129Sv background. Upon arrival at The Jackson Laboratory, mutant mice were bred to 129S1/SvImJ (Stock No. 002448) mice for at least one generation to establish the colony.

Expression Data

Expressed Gene	COMT, catechol-O-methyltransferase, human
Site of Expression	Human COMT is expressed in both prefrontal cortex and hippocampus.

Control Suggestions

Approximate Controls

002448 129S1/SvImJ

Additional Information

Considerations for Choosing Controls

Selected References

Risbrough V; Ji B; Hauger R; Zhou X. 2014. Generation and characterization of humanized mice carrying COMT158 Met/Val alleles. Neuropsychopharmacology 39(8):1823-32PubMed: 24509724 MGI: J:224284

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Genetics

Comt^{tm1(COMT)Xzho}

Allele Symbol: Comt^{tm1(COMT)Xzho}

Allele Name	targeted mutation 1, Xianjin Zhou
Allele Type	Targeted (Null/Knockout, Inserted expressed sequence)
Allele Synonym(s)	targeted mutation 1, Xianjin Zhou; Comt^{tm1(COMT)Xzho}
Gene Symbol and Name	Comt, catechol-O-methyltransferase
Gene Synonym(s)	Comt1; catechol-O-methyltransferase 1; DNA segment, Chr 16, Wayne State University 103, expressed; D330014B15Rik; D16Wsu103e; HEL-S-98n; RIKEN cDNA D330014B15 gene; Comt1; D16Wsu103e; D330014B15Rik
Expressed Gene	COMT, catechol-O-methyltransferase, human
Site of Expression	Human COMT is expressed in both prefrontal cortex and hippocampus.
Strain of Origin	129
Chromosome	16
Molecular Note	The targeting construct was designed to replace the entire endogenous coding region with the human sequence following by a neomycin (neo) resistance cassette. Western blot analysis confirmed the absence of protein expression in the hippocampus and cortex.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Behavioral and Learning Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Comt^{tm1(COMT)Xzho}/Comt^{tm1(COMT)Xzho}
involves: 129 * C57BL/6J

behavior/neurological phenotype

abnormal spatial working memory
- male and female mice exhibit reduced alternation compared with chance
- tolcapone treatment increases alteration unlike Comt^{tm2(COMT)Xzho} homozygotes

decreased vertical activity
- in female mice compared with female Comt^{tm2(COMT)Xzho} homozygotes

enhanced contextual conditioning behavior
- female mice exhibit increased freezing for the first 6 minutes of the test and then a decline unlike Comt^{tm2(COMT)Xzho} homozygotes

impaired cued conditioning behavior
- female mice exhibit decreased cued fear recall compared with Comt^{tm2(COMT)Xzho} homozygotes

increased startle reflex
- in male mice compared with male Comt^{tm2(COMT)Xzho} homozygotes

mammalian phenotype

behavior/neurological phenotype
- Normal - male mice exhibit normal exploratory behavior

nervous system phenotype

decreased prepulse inhibition
- in male mice compared with male Comt^{tm2(COMT)Xzho} homozygotes

increased prepulse inhibition
- in female mice compared with female Comt^{tm2(COMT)Xzho} homozygotes

References

Risbrough V; Ji B; Hauger R; Zhou X. 2014. Generation and characterization of humanized mice carrying COMT158 Met/Val alleles. Neuropsychopharmacology 39(8):1823-32PubMed: 24509724 MGI: J:224284

Additional - Comt^{tm1(COMT)Xzho} related

Technical Support

Contact Technical Support

Genotyping Protocols
- SEPARATED MELT: Comt^{tm#(COMT)Xzho}
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the Val/Val mouse strain in a publication, please cite the originating article(s) and include JAX stock #027990 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Comt<tm1(COMT)Xzho>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Human COMT Val, Val/Val

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Comttm1(COMT)Xzho

Allele Symbol: Comttm1(COMT)Xzho

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Comttm1(COMT)Xzho/Comttm1(COMT)Xzhoinvolves: 129 * C57BL/6J

behavior/neurological phenotype

mammalian phenotype

nervous system phenotype

References

Additional - Comttm1(COMT)Xzho related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Comt^{tm1(COMT)Xzho}

Allele Symbol: Comt^{tm1(COMT)Xzho}

Genotype: Comt^{tm1(COMT)Xzho}/Comt^{tm1(COMT)Xzho}
involves: 129 * C57BL/6J

Additional - Comt^{tm1(COMT)Xzho} related