B6(Cg)-Socs1^tm1.1Ayr/J

Stock number: 027985
Product name: SOCS1^KI
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

SOCS1^KI mice contain a mutation in the microRNA 155 target site of the Socs1 (suppressor of cytokine signaling 1) gene, making them useful when studying miRNA-target mRNA interactions in the immune system.

Detailed description

SOCS1^KI mice contain a mutation in the 3’ UTR of the Socs1 (suppressor of cytokine signaling 1) gene. SOCS1 is a negative regulator of multiple cytokines such as IL-2 and IFNγ. SOCS1 has been described as a target for miR-155 (microRNA 155), a prominent miRNA found in the immune system where it is involved in processes including proper lymphocyte development and maturation. This mutation affects the target site for miR-155. Knock-in mice have an increased amount of SOCS1 protein in isolated T cells and other cell types where miR-155 and SOCS1 are co-expressed, similar to that observed in miR-155-deficient T cells, and reduced Stat5 activation. The size of their Treg cell population is normal in contrast to the diminished population in miR-155-deficient mice. Homozygotes are viable and fertile.

Development

A targeting construct was designed by Alexander Rudensky (Memorial Sloan Kettering Cancer Center) to insert a loxP-flanked neomycin (neo) resistance cassette downstream of exon 1 of the suppressor of cytokine signaling 1 (Socs1) gene. Mutations (AGCTTAA to AGCAAATT) were introduced in the 3’ UTR, corresponding to the putative miR-155 (microRNA 155) target site, resulting in a non-sense mutation. This targeting construct was electroporated into B6(Cg)-Tyr^c-2J embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to C57BL/6J mice, and the offspring were subsequently bred to CAG-cre transgenic mice on a B6 background to remove the floxed selection cassette. SOCS1^KI mice were maintained on a C57BL/6 background by the donating investigator. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664). The Tyr^c-2J allele was not maintained in this line.

Allele

Symbol: Socs1^tm1.1Ayr
Name: targeted mutation 1.1, Alexander Y Rudensky
MGI accession ID: MGI:5698257
Generation method: Targeted
Attributes: Not Specified
Marker symbol: Socs1
Marker name: suppressor of cytokine signaling 1
Chromosome: 16
Strain of origin: B6(Cg)-Tyr^c-2J
Expressed genes: Socs1,suppressor of cytokine signaling 1,mouse, laboratory
Site of expression: Mice carrying this knock-in allele show an increased amount of SOCS1 protein in isolated T cells and other cell types where miR-155 and SOCS1 are co-expressed.
Molecular note: A targeting construct is designed to insert a loxP-flanked neomycin resistance cassette downstream of exon 1 of the gene. Mutations (AGCTTAA to AGCAAATT) were introduced in the 3' UTR, corresponding to the putative miR-155 (microRNA 155) target site, resulting in a non-sense mutation. Cre-mediated recombination removed the floxed neo cassette.