SOCS1KI mice contain a mutation in the microRNA 155 target site of the Socs1 (suppressor of cytokine signaling 1) gene, making them useful when studying miRNA-target mRNA interactions in the immune system.
Alexander Rudensky, Memorial Sloan Kettering Cancer Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Specified) | Socs1 | suppressor of cytokine signaling 1 |
SOCS1KI mice contain a mutation in the 3’ UTR of the Socs1 (suppressor of cytokine signaling 1) gene. SOCS1 is a negative regulator of multiple cytokines such as IL-2 and IFNγ. SOCS1 has been described as a target for miR-155 (microRNA 155), a prominent miRNA found in the immune system where it is involved in processes including proper lymphocyte development and maturation. This mutation affects the target site for miR-155. Knock-in mice have an increased amount of SOCS1 protein in isolated T cells and other cell types where miR-155 and SOCS1 are co-expressed, similar to that observed in miR-155-deficient T cells, and reduced Stat5 activation. The size of their Treg cell population is normal in contrast to the diminished population in miR-155-deficient mice. Homozygotes are viable and fertile.
A targeting construct was designed by Alexander Rudensky (Memorial Sloan Kettering Cancer Center) to insert a loxP-flanked neomycin (neo) resistance cassette downstream of exon 1 of the suppressor of cytokine signaling 1 (Socs1) gene. Mutations (AGCTTAA to AGCAAATT) were introduced in the 3’ UTR, corresponding to the putative miR-155 (microRNA 155) target site, resulting in a non-sense mutation. This targeting construct was electroporated into B6(Cg)-Tyrc-2J embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to C57BL/6J mice, and the offspring were subsequently bred to CAG-cre transgenic mice on a B6 background to remove the floxed selection cassette. SOCS1KI mice were maintained on a C57BL/6 background by the donating investigator. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664). The Tyrc-2J allele was not maintained in this line.
Expressed Gene | Socs1, suppressor of cytokine signaling 1, mouse, laboratory |
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Site of Expression | Mice carrying this knock-in allele show an increased amount of SOCS1 protein in isolated T cells and other cell types where miR-155 and SOCS1 are co-expressed. |
Allele Name | targeted mutation 1.1, Alexander Y Rudensky |
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Allele Type | Targeted (Not Specified) |
Allele Synonym(s) | SOCS1KI |
Gene Symbol and Name | Socs1, suppressor of cytokine signaling 1 |
Gene Synonym(s) | |
Expressed Gene | Socs1, suppressor of cytokine signaling 1, mouse, laboratory |
Site of Expression | Mice carrying this knock-in allele show an increased amount of SOCS1 protein in isolated T cells and other cell types where miR-155 and SOCS1 are co-expressed. |
Strain of Origin | B6(Cg)-Tyrc-2J |
Chromosome | 16 |
Molecular Note | A targeting construct is designed to insert a loxP-flanked neomycin resistance cassette downstream of exon 1 of the gene. Mutations (AGCTTAA to AGCAAATT) were introduced in the 3' UTR, corresponding to the putative miR-155 (microRNA 155) target site, resulting in a non-sense mutation. Cre-mediated recombination removed the floxed neo cassette. |
When maintaining a live colony homozygous mice may be bred together.
When using the SOCS1KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #027985 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Socs1<tm1.1Ayr> |
Frozen Mouse Embryo | B6(Cg)-Socs1<tm1.1Ayr>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Socs1<tm1.1Ayr>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Socs1<tm1.1Ayr>/J | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Socs1<tm1.1Ayr>/J | $3373.50 |
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