B6.Cg-Tg(Pbsn-KLKB1)1Gja/EkvaJ

Stock number: 027955
Product name: PSA-tg
Research areas: Developmental Biology Research, Internal/Organ Research, Virology Research

Description

PSA tg mice express human prostate specific antigen, KLKB1, under control of the probasin promoter, on a congenic B6 background. These mice are useful for studying CMV-based vectors as an effective anti-tumor immune response in a mouse model of prostate cancer.

Detailed description

PSA tg mice express human prostate specific antigen (KLKB1 kallikrein B, plasma 1, PSA) under control of the probasin (Pbsn) promoter. PSA is a serine protease produced at high levels by prostate epithelial cells and secreted into seminal plasma. PSA is a tumor marker for diagnosis and prognosis of prostatic carcinoma. Mice homozygous for the PSA tg allele are viable and fertile. Expression of PSA in the prostate was confirmed at the protein level by immunohistochemical staining with PSA-specific antibodies. Mice expressed PSA in ventral, dorsal and lateral prostate lobes, whereas the anterior prostate did not express detectable PSA. These mice have been developed to test the utility of mouse CMV (mCMV)-based vaccines expressing human prostate specific antigen (PSA) for prostate cancer immunotherapy.

Development

The PSA tg transgenic vector was generated with the human KLKB1 (kallikrein B, plasma 1 (also known as prostate specific antigen)) gene under control of a probasin (Pbsn). The construct was microinjected into fertilized oocytes, and founder mice were bred to C57BL/6J mice for at least 10 generations, and subsequently were inbred for at least 10 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Pbsn-KLKB1)1Gja
Name: transgene insertion 1, Gilbert Jay
MGI accession ID: MGI:5648385
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Pbsn-KLKB1)1Gja
Marker name: transgene insertion 1, Gilbert Jay
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: KLKB1,kallikrein B1,human
Molecular note: The transgenic vector was generated with the human KLKB1 (kallikrein B, plasma 1 (also known as prostate specific antigen)) gene under control of a probasin promoter.