These Abcg1flox mice have loxP sites flanking exon 3 of the Abcg1 gene. Removal of the floxed sequence creates a null allele. These mice may be useful in studying regulation of cellular cholesterol homeostasis and tumor immunity.
Catherine Hedrick, La Jolla Institute for Allergy and Immun
The Abcg1fl allele has loxP sites flanking exon 3 of the Abcg1 gene, and a frt site flanked NEO selection cassette downstream of the floxed exon 3.
The Abcg1 gene encodes an ATP-binding cassette (ABC) transporter from the white subfamily that is involved in macrophage cholesterol and phospholipids transport, lipid homeostasis regulation, and the antitumour immune response.
Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues. Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression in myeloid cells (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of cholesterol metabolism, atherosclerosis, and tumor immunity.
A targeting vector was designed by Dr. Catherine C. Hedrick (La Jolla Institute for Allergy and Immunology) to have a loxP site upstream of exon 3, and a second loxP site followed by an frt-flanked neo cassette just downstream of exon 3 of the ATP-binding cassette, sub-family G (WHITE), member 1 gene (Abcg1). The construct was electroporated into C57BL/6-derived iTL IC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Catherine Hedrick|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Abcg1, ATP binding cassette subfamily G member 1|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||The targeting vector was designed to have a loxP site upstream of exon 3, and a second loxP site followed by an FRT-flanked neo cassette downstream of exon 3.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Abcg1 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #027954 in your Materials and Methods section.