STOCK Tamm41^{tm1c(KOMP)Wtsi}/Mmjax

Stock number: 027948
Product name: Tamm41 flox
Research areas: Research Tools

Description

Exon 4 of the Tamm41 (mitochondrial translocator assembly and maintenance protein, homolog (S. cerevisiae)) gene is flanked by loxP sites in this conditional mutant strain. These mice may be useful in studies of mitochondrial cardiolipin.

Detailed description

This strain was generated using embryonic stem cells provided by the International Knockout Mouse Consortium.

In yeast, Tamm41 (mitochondrial translocator assembly and maintenance protein, homolog (S. cerevisiae)) encodes a mitochondrial CDP-diacylglycerol synthase involved in cardiolipin metabolism. Orthologous genes in human and mice have been identified and are predicted to be targeted to mitochondria. Exon 4 of the Tamm41 gene is flanked by loxP sites in this conditional mutant strain. Heterozygous and homozygous mice are healthy, appear normal and are fertile.

Development

The L1L2_Bact_P cassette was inserted at position 115025851 of Chromosome 6 upstream of exon 4. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4. The construct was introduced into C57BL/6N-derived JM8.A3N1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to CD1 females and progeny crossed to 129S4/SvJae-Gt(ROSA)26Sor^tm2(FLP*)Sor/J mice to remove the neomycin cassette. Mice were maintained on CD1 for an unknown number of generations. Upon arrival, mice were bred to C57BL/6NJ inbred mice for at least one generation to establish the colony.

Allele

Symbol: Tamm41^{tm1c(KOMP)Wtsi}
Name: targeted mutation 1c, Wellcome Trust Sanger Institute
MGI accession ID: MGI:5644834
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Tamm41
Marker name: TAM41 mitochondrial translocator assembly and maintenance homolog
Chromosome: 6
Strain of origin: C57BL/6N-A^tm1Brd
Molecular note: The L1L2_Bact_P cassette was inserted at position 115025851 of Chromosome 6 upstream of exon 4. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 4 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.