Overview

Also Known As:Tamm41 flox

Exon 4 of the Tamm41 (mitochondrial translocator assembly and maintenance protein, homolog (S. cerevisiae)) gene is flanked by loxP sites in this conditional mutant strain. These mice may be useful in studies of mitochondrial cardiolipin.

Donating Investigator

Roy Long, Medical College of Wisconsin

Genetic overview

Genetic Background	Generation

Tamm41^{tm1c(KOMP)Wtsi}

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Tamm41	TAM41 mitochondrial translocator assembly and maintenance homolog

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Research Applications

Research Tools

View All Research Applications

Details

Detailed Description

This strain was generated using embryonic stem cells provided by the International Knockout Mouse Consortium.

In yeast, Tamm41 (mitochondrial translocator assembly and maintenance protein, homolog (S. cerevisiae)) encodes a mitochondrial CDP-diacylglycerol synthase involved in cardiolipin metabolism. Orthologous genes in human and mice have been identified and are predicted to be targeted to mitochondria. Exon 4 of the Tamm41 gene is flanked by loxP sites in this conditional mutant strain. Heterozygous and homozygous mice are healthy, appear normal and are fertile.

Development

The L1L2_Bact_P cassette was inserted at position 115025851 of Chromosome 6 upstream of exon 4. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4. The construct was introduced into C57BL/6N-derived JM8.A3N1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to CD1 females and progeny crossed to 129S4/SvJae-Gt(ROSA)26Sor^tm2(FLP*)Sor/J mice to remove the neomycin cassette. Mice were maintained on CD1 for an unknown number of generations. Upon arrival, mice were bred to C57BL/6NJ inbred mice for at least one generation to establish the colony.

Control Suggestions

Approximate Controls

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Tamm41^{tm1c(KOMP)Wtsi}

Allele Symbol: Tamm41^{tm1c(KOMP)Wtsi}

Allele Name	targeted mutation 1c, Wellcome Trust Sanger Institute
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Tam41 flox
Gene Symbol and Name	Tamm41, TAM41 mitochondrial translocator assembly and maintenance homolog
Gene Synonym(s)
Strain of Origin	C57BL/6N-A^tm1Brd
Chromosome	6
Molecular Note	The L1L2_Bact_P cassette was inserted at position 115025851 of Chromosome 6 upstream of exon 4. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 4 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

References

Additional - Tamm41^{tm1c(KOMP)Wtsi} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tamm41-Alternate 1
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the Tamm41 flox mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41417 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Tamm41 flox

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Tamm41tm1c(KOMP)Wtsi

Allele Symbol: Tamm41tm1c(KOMP)Wtsi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tamm41tm1c(KOMP)Wtsi related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Tamm41^{tm1c(KOMP)Wtsi}

Allele Symbol: Tamm41^{tm1c(KOMP)Wtsi}

Additional - Tamm41^{tm1c(KOMP)Wtsi} related