STOCK Lats2^tm1.1Dupa/J

Stock number: 027934
Product name: Lats2flox
Research areas: Cancer Research, Developmental Biology Research, Research Tools

Description

Exons 5 and 6 of the mouse Lats2 gene are flanked by loxP sites in this Lats2^flox strain. Cre-mediated excision of the floxed region creates a knockout allele.

Detailed description

LATS2 (large tumor suppressor 2) phosphorylates YAP1 protein (see related Stock No. 027979) as part of the Hippo signaling pathway regulating growth, differentiation, and regeneration. Homozygous knockouts of Lats2 result in embryonic lethality.

Exons 5 and 6 of the mouse Lats2 gene are flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region creates a knockout allele.

When combined with a Lats1 gene knockout and treated with Ad-Cre to knock out Lats2, compound mutant mice develop massive hepatomegaly, with liver size reaching approximately 30% of the body weight 8 weeks after Ad-Cre delivery.

Development

A loxP site was placed in intron 4 and an FRT-neomycin-FRT-loxP cassette was introduced to intron 6 via homologous recombination in 129P2/OlaHsd-derived E14TG2a.4 embryonic stem (ES) cells. Resultant mice were crossed with a CMV-FLP recombinase-expressing strain on a C57BL/6 background to excise the neomycin cassette, leaving exons 5 and 6 flanked by loxP sites. This strain was maintained on a mixed C57BL/6-129 genetic background by the donating lab.

Allele

Symbol: Lats2^tm1.1Dupa
Name: targeted mutation 1.1, Duojia Pan
MGI accession ID: MGI:5648494
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Lats2
Marker name: large tumor suppressor 2
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Molecular note: A loxP site was placed in intron 4 and an FRT-neomycin-FRT-loxP cassette was introduced to intron 6. Flp-mediated recombination removed the FRT-flanked neo cassette.