Exons 5 and 6 of the mouse Lats2 gene are flanked by loxP sites in this Lats2flox strain. Cre-mediated excision of the floxed region creates a knockout allele.
Duojia Pan, UT Southwestern
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Lats2 | large tumor suppressor 2 |
LATS2 (large tumor suppressor 2) phosphorylates YAP1 protein (see related Stock No. 027979) as part of the Hippo signaling pathway regulating growth, differentiation, and regeneration. Homozygous knockouts of Lats2 result in embryonic lethality.
Exons 5 and 6 of the mouse Lats2 gene are flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region creates a knockout allele.
When combined with a Lats1 gene knockout and treated with Ad-Cre to knock out Lats2, compound mutant mice develop massive hepatomegaly, with liver size reaching approximately 30% of the body weight 8 weeks after Ad-Cre delivery.
A loxP site was placed in intron 4 and an FRT-neomycin-FRT-loxP cassette was introduced to intron 6 via homologous recombination in 129P2/OlaHsd-derived E14TG2a.4 embryonic stem (ES) cells. Resultant mice were crossed with a CMV-FLP recombinase-expressing strain on a C57BL/6 background to excise the neomycin cassette, leaving exons 5 and 6 flanked by loxP sites. This strain was maintained on a mixed C57BL/6-129 genetic background by the donating lab.
Allele Name | targeted mutation 1.1, Duojia Pan |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Lats2flox |
Gene Symbol and Name | Lats2, large tumor suppressor 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 14 |
Molecular Note | A loxP site was placed in intron 4 and an FRT-neomycin-FRT-loxP cassette was introduced to intron 6. Flp-mediated recombination removed the FRT-flanked neo cassette. |
Homozygous and heterozygous floxed mice are viable and fertile.
When using the Lats2flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #027934 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lats2<tm1.1Dupa> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.