APOBEC1 complementation factor (A1cf) mutant mice have a green fluorescent protein and a PGK-neo cassette replacing exon 2 of the A1cf gene, abolishing gene function. ACF is an RNA binding protein and cofactor for APOBEC1 cytidine deaminase in C-to-U RNA editing. A1CF protein is highly expressed in the kidney, liver, and small intestine of adult mice and humans, as well as in heart, spinal cord, and lung of mouse embryos (E12.5). A1CF transcripts were also detected in adult gonads in humans. A1cf+/- heterozygotes are are viable and fertile while homozygotes die before implantation (around E3.5), suggesting that A1CF is essential for early development. A1CF plays a crucial role in cell growth and differentiation, and in particular in liver regeneration. Indeed, A1cf+/- heterozygotes exhibit decreased proliferation and impaired liver mass restitution following partial hepatectomy. The donating investigator commented that it is unknown if the GFP is functional.

Acf+/- mice have a green fluorescent protein and a neo cassette replacing exon 2 of the APOBEC1 complementation factor (A1cf) gene. These mice may be useful for studying C-to-U RNA editing as well as cell survival during early embryonic development.

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