C57BL/6-Ccr7^tm1.1Dnc/J

Stock number: 027913
Product name: CCR7-GFP
Research areas: Research Tools

Description

Ccr7-gfp knockin/knockout mice serve as a reporter strain for migratory dendritic cells, naïve T cells, central memory T cells.

Detailed description

Ccr7 encodes a G protein-coupled chemokine receptor that is expressed in dendritic cells, naïve T cells, central memory T cells and B cells. CCR7 directs migration of these cells to lymphoid tissue, such as the spleen and lymph nodes. These Ccr7-gfp mice function as a reporter strain for migratory dendritic cells and naïve T cells. Ccr7/GFP expressing dendritic cells can be isolated and purified by flow cytometry. Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector containing loxP site flanked Neo cassette and sequence encoding Enhanced Green Fluorescent Protein was used to disrupt the coding region of the Ccr7 gene. The construct was electroporated into C57BL/6NTac derived B8/F1 embryonic stem (ES) cells which were transiently transfected with a cre-expressing plasmid DNA to remove the selection cassette. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to C57BL/6J for 2 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Ccr7^tm1.1Dnc
Name: targeted mutation 1.1, Donald Cook
MGI accession ID: MGI:5660365
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Ccr7
Marker name: chemokine (C-C motif) receptor 7
Chromosome: 11
Strain of origin: C57BL/6NTac
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in dendritic cells.
Molecular note: The targeting vector contains a loxP-flanked neomycin cassette and sequence encoding Enhanced Green Fluorescent Protein was used to disrupt the coding region of the Ccr7 gene. Transient transfection with a cre-expressing plasmid DNA removed the selection cassette.