Ccr7-gfp knockin/knockout mice serve as a reporter strain for migratory dendritic cells, naïve T cells, central memory T cells.
Donald N. Cook, NIH/NIEHS
Genetic Background | Generation |
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N2+pN1F13
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Ccr7 | chemokine (C-C motif) receptor 7 |
Ccr7 encodes a G protein-coupled chemokine receptor that is expressed in dendritic cells, naïve T cells, central memory T cells and B cells. CCR7 directs migration of these cells to lymphoid tissue, such as the spleen and lymph nodes. These Ccr7-gfp mice function as a reporter strain for migratory dendritic cells and naïve T cells. Ccr7/GFP expressing dendritic cells can be isolated and purified by flow cytometry. Mice that are homozygous for the targeted mutation are viable and fertile.
A targeting vector containing loxP site flanked Neo cassette and sequence encoding Enhanced Green Fluorescent Protein was used to disrupt the coding region of the Ccr7 gene. The construct was electroporated into C57BL/6NTac derived B8/F1 embryonic stem (ES) cells which were transiently transfected with a cre-expressing plasmid DNA to remove the selection cassette. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to C57BL/6J for 2 generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | GFP is expressed in dendritic cells. |
Allele Name | targeted mutation 1.1, Donald Cook |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Ccr7gfp |
Gene Symbol and Name | Ccr7, chemokine (C-C motif) receptor 7 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in dendritic cells. |
Strain of Origin | C57BL/6NTac |
Chromosome | 11 |
Molecular Note | The targeting vector contains a loxP-flanked neomycin cassette and sequence encoding Enhanced Green Fluorescent Protein was used to disrupt the coding region of the Ccr7 gene. Transient transfection with a cre-expressing plasmid DNA removed the selection cassette. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the CCR7-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #027913 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ccr7<tm1.1Dnc> |
Frozen Mouse Embryo | C57BL/6-Ccr7<tm1.1Dnc>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Ccr7<tm1.1Dnc>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Ccr7<tm1.1Dnc>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Ccr7<tm1.1Dnc>/J | $3373.50 |
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