Overview

Also Known As:NSG.PDL1-

PDL1-/-.NOD NSG mice are NOD/LtJ-congenic animals carrying the PD-L1^- (Cd274^-) knockout mutation that have been backcrossed to NSG mice (Stock No. 005557).

Donating Investigator

Arlene H Sharpe, Harvard Medical School

Genetic overview

Genetic Background	Generation

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Cd274	CD274 antigen

Research Applications

Virology Research
Cancer Research
Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research
Cell Biology Research
Hematological Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

The signal and all of the immunoglobulin (Ig)-V-like exon of the CD274 antigen (Cd274) gene were replaced with a neomycin resistance cassette, abolishing PD-L1 gene expression. PD-L1 is one of two ligands for PD-1 and is upregulated on antigen presenting cells (APCs), activated T cells, myeloid cells, dendritic cells (DCs). It is also expressed on hematopoietic and parenchymal cells. PD-1 is an inhibitory receptor expressed on activated lymphocytes, regulates tolerance and autoimmunity by negative regulation of T cell responses. On the NSG background, mice should be immunodeficient. They should have no mature T cells or B cells, lack functional natural killer (NK) cells, have reduced numbers of lymphocytes and myeloid dendritic cells, and be deficient in cytokine signaling.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these congenic mice could vary from that originally described. We may modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to replace the signal and all of the immunoglobulin (Ig)-V-like exon of the CD274 antigen (Cd274) gene with a neomycin resistance cassette. This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred together to generate a colony of PD-L1- mice. The mutant mice were then backcrossed with NOD/LtJ inbred mice for at least 15 generations. The donating investigator reports that these mice are confirmed homozygous for Idd 1, 2, 3, 4, 5a, 5d, 7, 8, 9, 10, 11, 12, 13, 14, 15 loci after speed congenic backcross to NOD/LtJ. Upon arrival at The Jackson Laboratory Repository, mice were bred to NOD/ShiLtJ inbred mice (Stock No. 001976) for at least one generation and are maintained as 018307. Some mice were also backcrossed to NSG mice (Stock No. 005557) and are maintained as this Stock No. 027905.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Cd274^tm1Shr

Allele Symbol: Cd274^tm1Shr

Allele Name	targeted mutation 1, Arlene H Sharpe
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	PD-L1^-
Gene Symbol and Name	Cd274, CD274 antigen
Gene Synonym(s)
Strain of Origin	C57BL/6
Chromosome	19
Molecular Note	The signal and all of the IgV exon was replaced with a neomycin resistance gene. Southern blot confirmed recombination. Flow cytometry showed lack of expression in B cells, T cells, macrophages or DCs in mutants.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdc^scid related

Virology Research
- B and T Cell Deficiency
  - AIDS research tool
Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency

Genotype: Il2rg^tm1Wjl related

Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Virology Research
- B and T Cell Deficiency
Research Tools
- Cell Biology Research
- Cancer Research
  - T cell deficiency
  - specific T cell deficiency
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Deficiency
  - T cell deficiency
  - specific T cell deficiency
Cell Biology Research
- Transcriptional Regulation
- Genes Regulating Growth and Proliferation
- Signal Transduction
- Cell Cycle Regulation
Cancer Research
- Other
- Genes Regulating Growth and Proliferation
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - specific T cell deficiency
  - T cell deficiency
  - B and T cell deficiency
  - Asthma
- Autoimmunity
  - collagen induced arthritis
  - experimental allergic encephalomyelitis (EAE)
- Intracellular Signaling Molecules
- T Cell Receptor Signaling Defects
- Growth Factors/Receptors/Cytokines
- Inflammation
  - Asthma
  - B and T cell deficiency
- Lymphoid Tissue Defects
  - B and T cell deficiency
Internal/Organ Research
- Lymphoid Tissue Defects
  - T cell deficiency
- Thymus Defects
  - B and T cell deficient
Hematological Research
- Immunological Defects
  - B and T cell deficiency

Mammalian Phenotype Terms by Genotype

References

Additional - Cd274^tm1Shr related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Il2rg-PROBE
- End Point Analysis:Prkdc End Point
- Separated PCR:Cd274-Alternate 3
- Probe:Cd274 Probe
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining the live congenic colony, females that are homozygous for Prkdc^scid and Il2rg^tm1Wjl are bred to males that are homozygous for Prkdc^scid and hemizygous for Il2rg^tm1Wjl (Il2rg is an X-linked gene). Cd274^tm1Shr allele should be maintained as heterozygous in the colony due to lethality issues.
- Additional Breeding and Husbandry Support
Citation
When using the NSG.PDL1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #027905 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Homozygous for Prkdc<scid>, heterozygous or wildtype for Cd274<tm1Shr>, homoygous females and hemizygous male for Il2rg<tm1Wjl>

Related Products and Services

Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Cd274<tm1Shr> Il2rg<tm1Wjl>/J	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Cd274<tm1Shr> Il2rg<tm1Wjl>/J	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Cd274<tm1Shr> Il2rg<tm1Wjl>/J	$3373.50
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Cd274<tm1Shr> Il2rg<tm1Wjl>/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

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Animal Care and Use for SERVICES

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As:NSG.PDL1-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Prkdcscid

Allele Symbol: Prkdcscid

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Cd274tm1Shr

Allele Symbol: Cd274tm1Shr

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Genotype: Il2rgtm1Wjl related

Mammalian Phenotype Terms by Genotype

References

Additional - Cd274tm1Shr related

Additional - Il2rgtm1Wjl related

Additional - Prkdcscid related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Prkdc^scid

Allele Symbol: Prkdc^scid

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Cd274^tm1Shr

Allele Symbol: Cd274^tm1Shr

Genotype: Prkdc^scid related

Genotype: Il2rg^tm1Wjl related

Additional - Cd274^tm1Shr related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related