B6.Cg-Tg(Msx2-cre)5Rem/BortJ

Stock number: 027892
Product name: Msx2-Cre
Research areas: Developmental Biology Research, Research Tools

Description

These mice express Cre recombinase under the control of the Msx2 promoter in in secondary follicles and in apical ectodermal ridge cells of both forelimb and hindlimb buds.

Detailed description

These mice express Cre recombinase under the control of the murine msh homeobox 2 (Msx2) promoter. MSX2 acts as a transcriptional repressor during during both embryogenesis and postnatal development. During organogenesis MSX2 is expressed specifically during the development of the skull, teeth, and skin. Expression is also seen in prospective apical ectodermal ridge (AER) cells of both forelimb and hindlimb buds, and during postnatal oogenesis in secondary follicles. Hemizygotes are viable and fertile. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in cre-expressing tissues.

Of note, these mice express Cre recombinase at a later stage of folliculogenesis than C57BL/6-Tg(Zp3-cre)93Knw/J mice (Stock No. 003651) which expresses in primary follicles.

Development

A transgenic construct containing cre coding sequence under the control of the msh homeobox 2 (Msx2) promoter was introduced into B6CBA donor oocytes. Resulting offspring from founder line 5 were bred to CD1 outbred mice, and were subsequently bred to C57BL/6J mice for at least 20 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Msx2-cre)5Rem
Name: transgene insertion 5, Robert E Maxson
MGI accession ID: MGI:2159109
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Msx2-cre)5Rem
Marker name: transgene insertion 5, Robert E Maxson
Chromosome: UN
Strain of origin: C57BL/6 x CBA
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is expressed in secondary follicles and in apical ectodermal ridge cells of both forelimb and hindlimb buds.
Molecular note: This transgene expresses Cre recombinase under the control of the Msx2 promoter in the embryonic limb regions and in the nascent AER.