STOCK Tg(Actl6b-Cre)4092Jiwu/J

Stock number: 027826
Product name: BAF53b-Cre
Research areas: Neurobiology Research, Research Tools

Description

These BAF53b-Cre transgenic mice express Cre recombinase under the control of the mouse Actl6b gene promoter to provide pan-neuronal cre recombinase activity.

Detailed description

The actin-related protein encoded by the Actl6b gene (also known as BAF53b) is a subunit of the neuron-specific chromatin remodeling BRG1/brm-associated factor (BAF) complexes. These BAF53b-Cre transgenic mice express Cre recombinase under the control of the mouse Actl6b gene promoter. Cre recombinase expression is detected in the cerebellum and cortex of the brain. Lower levels of Cre recombinase activity is detected in the eye and testis. At E13.5, there is Cre recombinase activity observed in brain and spinal cord, as well as the peripheral nervous system (dorsal root ganglion, sympathetic ganglia, nerve tracts). In postnatal brains of transgenic mice, Cre activity is found in neurons, but not in proliferating neural progenitor cells or glial cells. Cre recombinase activity is also identified in primary cultures of hippocampal and cortical neurons. The Donating Investigator reports that the transgene did not insert into the X chromosome, and that there is no difference in phenotype between hemizygotes and homozygotes. Mice that are homozygous for the transgene are viable and fertile.

Development

The mouse bacterial artificial chromosome (BAC) RP23-127E17 containing the entire Actl6b gene and approximately 100 kb flanking sequence, was modified by replacing the first 2 exons of the Actl6b gene with the PCR product of the Cre recombinase gene and Actl6b 3’ UTR, which situates the cre gene just downstream of the start codon. DNA from the modified BAC was used to prepare the BAF53b-Cre transgene which was then microinjected into the pronucleus of FVB/N fertilized eggs. Founder line 4092 was subsequently established. The mice were then crossed to other mutant strains on various genetic backgrounds. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(Actl6b-cre)4092Jiwu
Name: transgene insertion 4092, Jie Wu
MGI accession ID: MGI:5699179
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Actl6b-cre)4092Jiwu
Marker name: transgene insertion 4092, Jie Wu
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase expression is detected in the cerebellum and cortex of the brain.
Molecular note: The mouse bacterial artificial chromosome (BAC) RP23-127E17 containing the entire Actl6b gene and approximately 100 kb flanking sequence, was modified by replacing the first 2 exons of the Actl6b gene with the PCR product of the cre recombinase gene and Actl6b 3' UTR, which situates the cre gene just downstream of the start codon. Founder line 4092 was subsequently established.