B6;CBA-Tg(Emx1-cre/ERT2)1Kess/SshiJ

Stock number: 027784
Product name: Emx1-CreER^T2
Research areas: Neurobiology Research, Research Tools

Description

Emx1-Cre-ER^T2 mice express Cre in pallial precursors cells making them useful for applications requiring tamoxifen-induced deletion of floxed sequences in these cells.

Detailed description

Mice hemizygous for the PAC Emx1-Cre-ER^T2 transgene are viable and fertile. These mice express Cre protein directed by the endogenous Emx1 (empty spiracles homeobox 1) promoter/enhancer regions on the PAC transgene. Cre expression is seen in pallial precursors cells. Cre-ER^T2 is restricted to the cytoplasm and can only gain access to the nuclear compartment after exposure to tamoxifen. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.

Development

A P1-derived artificial chromosome (PAC) containing the entire mouse Emx1 (empty spiracles homeobox 1) gene was modified by targeted mutation to fuse the CreER^T2 gene to the ATG of Emx1. This was followed by a SV40 polyadenylation signal. This construct was introduced into C57BL6/CBA F1 donor oocytes. Resulting offspring were maintained by breeding to C57BL6/CBA F1 mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Emx1-cre/ERT2)1Kess
Name: transgene insertion 1, Nicoletta Kessaris
MGI accession ID: MGI:3761168
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Marker symbol: Tg(Emx1-cre/ERT2)1Kess
Marker name: transgene insertion 1, Nicoletta Kessaris
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Cre-ERT2 fusion protein is expressed from endogenous Emx1 promoter/enhancer elements in the developing brain.
Molecular note: A P1 artificial chromosome was created that contained a tamoxifen-inducible Cre recombinase with a nuclear localization signal fused to the initiation codon. Expression recapitulates endogenous expression in the cortical precursors.