The coding region of the mouse Sox17 gene is flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region results in a knockout allele that expresses human placental alkaline phosphatase (AP).
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Reporter) | Sox17 | SRY (sex determining region Y)-box 17 |
Sox17 (SRY (sex determining region Y)-box 17) encodes a transcription factor involved with the regulation of embryonic development that is expressed in vascular endothelial cells.
Floxed animals in the colony maintained by the donating laboratory demonstrated no overt phenotype. Cre-mediated excision of the floxed region results in a knockout allele that expresses human placental alkaline phosphatase (AP). Germline recombination leads to mid-embryonic lethality due to defects in cardiovascular/hematopoietic development.
Homozygotes maintained in the colony at The Jackson Laboratory exhibit progressive polyuria starting around 7 weeks of age, but the mice are not diabetic (as determined by fed-glucose levels). Their insulin/pro-insulin levels are normal, and retired breeders have normal pancreas pathology (according to our board-certified veterinary pathology).
The amount of urine produced can become so severe that staff must change cages every 24 hours. Given the severity of this phenotype, we will no longer be offering homozygotes from this strain. Investigators maintaining homozygous colonies should ensure that their bedding is being changed at the proper frequency. Please note that heterozygous mice do not exhibit polyuria.
An alternative Sox17 floxed mutation is offered in Stock No. 031712
LoxP sites were inserted (1) within the first coding exon 5’ of the initiator methionine codon, and (2) 3’ of an SV40 polyadenylation signal that was inserted immediately 3’ of the 3’ UTR. The following DNA segments were added immediately downstream of the 3’ loxP site: (1) the coding region for human placental alkaline phosphatase (AP), and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by frt sites (FNF). The targeting construct was electroporated into 129-derived ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice and then germline Flp mice (see Stock No. 003800) to excise the frt-PGK-Neo-frt (FNF) cassette. This strain was backcrossed to C57BL/6J for 3 generations by the donating laboratory.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
---|---|
Site of Expression | Vascular endothelial cells |
Allele Name | targeted mutation 1.1, Jeremy Nathans |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Sox17, SRY (sex determining region Y)-box 17 |
Gene Synonym(s) | |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Site of Expression | Vascular endothelial cells |
Strain of Origin | 129 |
Chromosome | 1 |
Molecular Note | LoxP sites are inserted within the first coding exon 5' of the initiator methionine codon, and 3' of an SV40 polyadenylation signal that is inserted immediately 3' of the 3' UTR. The following is downstream of the 3' loxP site: the coding region for human placental alkaline phosphatase (AP), and an FRT-flanked phosphoglycerate kinase (PGK)-Neo cassette (FNF). |
Floxed homozygotes and heterozygotes are viable and fertile.
When using the Sox17 CKO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027712 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Sox17<tm1.1Nat> |
Frozen Mouse Embryo | STOCK Sox17<tm1.1Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sox17<tm1.1Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sox17<tm1.1Nat>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Sox17<tm1.1Nat>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.