STOCK Sox17^tm1.1Nat/J

Stock number: 027712
Product name: Sox17 CKO
Research areas: Cell Biology Research, Research Tools

Description

The coding region of the mouse Sox17 gene is flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region results in a knockout allele that expresses human placental alkaline phosphatase (AP).

Detailed description

Sox17 (SRY (sex determining region Y)-box 17) encodes a transcription factor involved with the regulation of embryonic development that is expressed in vascular endothelial cells.

Floxed animals in the colony maintained by the donating laboratory demonstrated no overt phenotype. Cre-mediated excision of the floxed region results in a knockout allele that expresses human placental alkaline phosphatase (AP). Germline recombination leads to mid-embryonic lethality due to defects in cardiovascular/hematopoietic development.

Homozygotes maintained in the colony at The Jackson Laboratory exhibit progressive polyuria starting around 7 weeks of age, but the mice are not diabetic (as determined by fed-glucose levels). Their insulin/pro-insulin levels are normal, and retired breeders have normal pancreas pathology (according to our board-certified veterinary pathology).

The amount of urine produced can become so severe that staff must change cages every 24 hours. Given the severity of this phenotype, we will no longer be offering homozygotes from this strain. Investigators maintaining homozygous colonies should ensure that their bedding is being changed at the proper frequency. Please note that heterozygous mice do not exhibit polyuria.

An alternative Sox17 floxed mutation is offered in Stock No. 031712

Development

LoxP sites were inserted (1) within the first coding exon 5’ of the initiator methionine codon, and (2) 3’ of an SV40 polyadenylation signal that was inserted immediately 3’ of the 3’ UTR. The following DNA segments were added immediately downstream of the 3’ loxP site: (1) the coding region for human placental alkaline phosphatase (AP), and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by frt sites (FNF). The targeting construct was electroporated into 129-derived ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice and then germline Flp mice (see Stock No. 003800) to excise the frt-PGK-Neo-frt (FNF) cassette. This strain was backcrossed to C57BL/6J for 3 generations by the donating laboratory.

Allele

Symbol: Sox17^tm1.1Nat
Name: targeted mutation 1.1, Jeremy Nathans
MGI accession ID: MGI:5638079
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Sox17
Marker name: SRY (sex determining region Y)-box 17
Chromosome: 1
Strain of origin: 129
Expressed genes: ALPP,alkaline phosphatase, placental,human
Site of expression: Vascular endothelial cells
Molecular note: LoxP sites are inserted within the first coding exon 5' of the initiator methionine codon, and 3' of an SV40 polyadenylation signal that is inserted immediately 3' of the 3' UTR. The following is downstream of the 3' loxP site: the coding region for human placental alkaline phosphatase (AP), and an FRT-flanked phosphoglycerate kinase (PGK)-Neo cassette (FNF).