STOCK Sox7^tm1.1Nat/J

Stock number: 027711
Product name: Sox7 CKO
Research areas: Cell Biology Research, Research Tools

Description

The coding region of the mouse Sox7 gene is flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region results in a knockout allele that expresses cyan fluorescent protein (CFP) and hemagglutinin tags.

Detailed description

Sox7 (SRY (sex determining region Y)-box 7) encodes a transcription factor involved with the regulation of embryonic development that is expressed in vascular endothelial cells.

The coding region of the mouse Sox7 gene is flanked by loxP sites in this conditional mutant strain. Floxed animals demonstrate no overt phenotype. Cre-mediated excision of the floxed region results in a knockout allele that expresses cyan fluorescent protein (CFP) and hemagglutinin tags. Germline recombination leads to mid-embryonic lethality due to defects in cardiovascular/hematopoietic development.

Development

LoxP sites were inserted (1) within the first exon 5’ of the initiator methionine codon, and (2) 3’ of an SV40 polyadenylation signal that was inserted immediately 3’ of the 3’ UTR. The following DNA segments were added immediately downstream of the 3’ loxP site: (1) the coding region for CFP with a 3×HA tag, and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by frt sites (FNF), where a third loxP site was inserted immediately 5’ of the second frt site. The targeting construct was electroporated into 129-derived ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice and then germline Flp mice (see Stock No. 003800) to excise the frt-PGK-Neo-frt (FNF) cassette. This strain was backcrossed to C57BL/6J for 3 generations by the donating lab.

Allele

Symbol: Sox7^tm1.1Nat
Name: targeted mutation 1.1, Jeremy Nathans
MGI accession ID: MGI:5638074
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Sox7
Marker name: SRY (sex determining region Y)-box 7
Chromosome: 14
Strain of origin: 129
Expressed genes: HA tag,HA tag,; CFP,Cyan Fluorescent Protein,jellyfish
Site of expression: Sox7 is expressed in vascular endothelial cells.
Molecular note: LoxP sites are inserted (1) within the first exon 5' of the initiator methionine codon, and (2) 3' of an SV40 polyadenylation signal that is inserted immediately 3' of the 3' UTR. Inserted downstream of the 3' loxP site are the coding region for CFP with a 3' HA tag, an FRT site, a phosphoglycerate kinase (PGK)-Neo cassette, a third loxP site and an FRT site.