C57BL/6J-Tg(Tcra,Tcrb)6482Dmky/J

Stock number: 027708
Product name: BT-II
Strain synonyms: Blo t 5 CD4 TCR transgenic mice
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

T cells from these TCR transgenic mice recognize the Blo t 5 antigen from Blomia tropicalis. These BT-II mice are useful in studies of allergic lung inflammation associated with acute asthma.

Detailed description

Blomia tropicalis is the predominant mite species in tropical and subtropical regions, the 4th most common mite in the United States, and an important source of aeroallergens. Blo t 5, the major Blomia tropicalis allergen, provokes a very high rate of sensitization. These BT-II TCR transgenic mice express the mouse α-chain and β-chain T cell receptor which recognizes peptide 55–70 from the Blo t 5 antigen from Blomia tropicalis under the control of the natural TCRα and TCRβ promoter/enhancer elements. More than 98% CD4 T cells coexpress specific Vα 11.1/11.2 and Vβ3, as assessed by flow cytometry of peripheral blood from transgenic mice.When Blo t 5-specific T cells from these transgenic mice are transplanted to naïve mice, intranasal antigen challenge induces airway inflammation and hyperresponsiveness in the lung. Mice homozygous for the transgenic insert are viable and fertile.

Development

Rearranged Blo t 5 peptide 55–70 specific T cell VαJα and VβD&betaJβ genes were cloned into pTα and pTβ cassettes. Transgenic constructs generated from these cassette vectors, containing the α-chain and β-chain of the T-cell receptor from a T cell hybridoma clone including the endogenous TCR promoter were coinjected into fertilized C57BL/6J mouse eggs. Founder line 6482 mice were then bred to wild-type C57BL/6J mice for at least 8 generations prior to arrival at The Jackson’s Laboratory. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(Tcra,Tcrb)6482Dmky
Name: transgene insertion 6482, David M Kemeny
MGI accession ID: MGI:5816373
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Tcra,Tcrb)6482Dmky
Marker name: transgene insertion 6482, David M Kemeny
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Site of expression: T cells.
Molecular note: Rearranged Blo t 5 peptide 55-70 (from the storage mite Blomia tropicalis) specific T cell ValphaJalpha and VbetaD&betaJbeta genes were cloned into pTalpha and pTbeta cassettes. Transgenic constructs generated from these cassette vectors, contain the alpha-chain and beta-chain of the T-cell receptor from a T cell hybridoma clone>