This triple mutant line was generated by intercrossing the single mutant lines to obtain triple heterozygotes with all 3 alleles in cis on chromosome 11. Mice that underwent meiotic recombination events placing all 3 mutations on the same homolog of chromosome 11 (in cis) were selected.


For the Trp53^tm1Tyj allele: a targeting vector was designed to replace approximately 40% of the coding sequences (involving exons 2-6) with a neomycin resistance cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. It is not known if the mice were backcrossed to C57BL/6 mice. The Donating Investigator obtained the mice from The Jackson Laboratory.



For the Nf1^tm1Tyj allele: a targeting vector was use to replace the first 42 codons of exon 31, the exon 31 splice acceptor site, and approximately 2 kb of intron 30 with a neomycin resistance cassette (oriented in an opposite transcriptional direction). The vector was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. This strain was obtained by the Donating Investigator from Dr. Tyler Jacks. The mice were backcrossed to C57BL/6 background more than twelve times by the donating laboratory.



For the Suz12^{Gt(Betageo)1Khe}allele: gene trap vector containing β-GEO
was inserted into intron 7, generating a truncation product of 276 N-terminal SUZ12 amino acids fused to 1323 amino acids of the β-GEO (beta-galactosidase-neomycin) protein. The vector was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice. It is not known if the mice were backcrossed to C57BL/6 mice. The Donating Investigator obtained the mice from Dr. Kristian Helin.



Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

• 101045 B6129SF2/J

Additional Information

• Considerations for Choosing Controls

• De Raedt T; Beert E; Pasmant E; Luscan A; Brems H; Ortonne N; Helin K; Hornick JL; Mautner V; Kehrer-Sawatzki H; Clapp W; Bradner J; Vidaud M; Upadhyaya M; Legius E; Cichowski K. 2014. PRC2 loss amplifies Ras-driven transcription and confers sensitivity to BRD4-based therapies. Nature 514(7521):247-51PubMed: 25119042MGI: J:217077