These Glowing Head transgenic mice on an albino C57BL/6 background are immunologically tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as a host for labeled syngeneic tumor transplantation.
Dr. Glenn T. Merlino, National Institutes of Health
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Tyr | tyrosinase |
Allele Type |
---|
Transgenic (Reporter) |
These “Glowing Head” (GH) transgenic mice express the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter. Transgene expression is targeted to the non-immune privileged anterior pituitary and does not interfere with pituitary function, as assessed by serum growth hormone levels. Bioluminescence is detected in the head and testes by in vivo imaging. Antigenicity of xenobiotic makers, including luciferase and GFP, causes
decrease in both the growth and metastatic potential of the labeled tumor in immunocompetent mice.
These transgenic mice are immune-tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as an allograft recipient for labeled syngeneic tumor transplantation, avoiding the effects of xenobiotic/antigenic reporters on testing therapeutic agents.
Metastases originating from transplanted ffLuc-eGFP-labeled cancer cells or tumors are increased in GH mice compared to similarly treated wildtype controls and BALB/c background NOD/SCID mice (see Stock No. 017619).
Mice that are homozygous for the transgene are viable and fertile. Homozygous mice may have a two-fold increase in head bioluminescence intensity.
This “Glowing Head” transgenic is also available on a FVB/N genetic background: Stock No. 027665, and a BALB/c genetic background: Stock No. 027848.
A transgenic construct containing the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter was injected into C57BL/6 blastocysts. Founder line D8 animals were bred to B6N-Tyrc-Brd/BrdCrCrl mice. The strain was maintained as albino. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | luc, luciferase, firefly |
Site of Expression | The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes. |
Allele Name | albino, Allan Bradley |
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Allele Type | Spontaneous |
Allele Synonym(s) | cBrd; C57BL/6c- |
Gene Symbol and Name | Tyr, tyrosinase |
Gene Synonym(s) | |
Site of Expression | Melanocytes. |
Strain of Origin | C57BL/6 |
Chromosome | 7 |
Molecular Note | This mutation arose spontaneously in or around 1991 in the C57BL/6 colony of Dr. Alan Bradley. It has been used in linkage studies based on the location of the tyrosinase gene on Chr 7, and its phenotype is complemented by a tyrosinase mini-gene. Sequence analysis of exon 1 identified the same G-to-T transversion at coding nucleotide 230 (c.230G>T), resulting in replacement of arginine by leucine at amino acid position 77 (p.R77L), that is present in the albino 2 Jackson allele Tyrc-2J. It reverts at a very low frequency (there were two incidences in the original colony between 1992 and 1995), resulting in black pups in otherwise albino litters. |
Allele Name | transgene insertion D8, Glenn Merlino |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | GH; Glowing Head; rGH-ffLuc-eGFP |
Gene Symbol and Name | Tg(Gnrhr-luc/EGFP)D8Mrln, transgene insertion D8, Glenn Merlino |
Gene Synonym(s) | |
Promoter | Gnrhr, gonadotropin releasing hormone receptor, rat |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The transgenic construct contains a firefly luciferase (ffLuc)-eGFP fusion gene under the control of the rat growth hormone gene promoter and human growth hormone sequences. Reporter activity (by bioluminescent imaging) is detected in the anterior pituitary gland and testes. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Glowing Head on B6 mouse strain in a publication, please cite the originating article(s) and include JAX stock #027662 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tyr<c-Brd>, Hemizygous for Tg(Gnrhr-luc/EGFP)D8Mrln |
Frozen Mouse Embryo | C57BL/6-Tyr<c-Brd> Tg(Gnrhr-luc/EGFP)D8Mrln/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tyr<c-Brd> Tg(Gnrhr-luc/EGFP)D8Mrln/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tyr<c-Brd> Tg(Gnrhr-luc/EGFP)D8Mrln/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tyr<c-Brd> Tg(Gnrhr-luc/EGFP)D8Mrln/J | $3373.50 |
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