Overview

Also Known As:Glowing Head on B6

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers who register interest will be contacted when the strain is again available.

These Glowing Head transgenic mice on an albino C57BL/6 background are immunologically tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as a host for labeled syngeneic tumor transplantation.

Donating Investigator

Dr. Glenn T. Merlino, National Institutes of Health

Genetic overview

Genetic Background	Generation

Tyr^c-Brd

Allele Type	Gene Symbol	Gene Name
Spontaneous	Tyr	tyrosinase

Tg(Gnrhr-luc/EGFP)D8Mrln

Allele Type
Transgenic (Reporter)

View Genetics

Research Applications

Research Tools
Cancer Research

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Details

Detailed Description

These “Glowing Head” (GH) transgenic mice express the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter. Transgene expression is targeted to the non-immune privileged anterior pituitary and does not interfere with pituitary function, as assessed by serum growth hormone levels. Bioluminescence is detected in the head and testes by in vivo imaging. Antigenicity of xenobiotic makers, including luciferase and GFP, causes
decrease in both the growth and metastatic potential of the labeled tumor in immunocompetent mice.
These transgenic mice are immune-tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as an allograft recipient for labeled syngeneic tumor transplantation, avoiding the effects of xenobiotic/antigenic reporters on testing therapeutic agents.
Metastases originating from transplanted ffLuc-eGFP-labeled cancer cells or tumors are increased in GH mice compared to similarly treated wildtype controls and BALB/c background NOD/SCID mice (see Stock No. 017619).

Mice that are homozygous for the transgene are viable and fertile. Homozygous mice may have a two-fold increase in head bioluminescence intensity.

This “Glowing Head” transgenic is also available on a FVB/N genetic background: Stock No. 027665, and a BALB/c genetic background: Stock No. 027848.

Development

A transgenic construct containing the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter was injected into C57BL/6 blastocysts. Founder line D8 animals were bred to B6N-Tyr^c-Brd/BrdCrCrl mice. The strain was maintained as albino. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	luc, luciferase, firefly
Site of Expression	The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Day CP; Carter J; Weaver Ohler Z; Bonomi C; El Meskini R; Martin P; Graff-Cherry C; Feigenbaum L; Tuting T; Van Dyke T; Hollingshead M; Merlino G. 2014. "Glowing head" mice: a genetic tool enabling reliable preclinical image-based evaluation of cancers in immunocompetent allografts. PLoS One 9(11):e109956PubMed: 25369133 MGI: J:221565

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Genetics

Tyr^c-Brd

Allele Symbol: Tyr^c-Brd

Allele Name	albino, Allan Bradley
Allele Type	Spontaneous
Allele Synonym(s)	c^Brd; C57BL/6^c-
Gene Symbol and Name	Tyr, tyrosinase
Gene Synonym(s)
Site of Expression	Melanocytes.
Strain of Origin	C57BL/6
Chromosome	7
Molecular Note	This mutation arose spontaneously in or around 1991 in the C57BL/6 colony of Dr. Alan Bradley. It has been used in linkage studies based on the location of the tyrosinase gene on Chr 7, and its phenotype is complemented by a tyrosinase mini-gene. Sequence analysis of exon 1 identified the same G-to-T transversion at coding nucleotide 230 (c.230G>T), resulting in replacement of arginine by leucine at amino acid position 77 (p.R77L), that is present in the albino 2 Jackson allele Tyr^c-2J. It reverts at a very low frequency (there were two incidences in the original colony between 1992 and 1995), resulting in black pups in otherwise albino litters.

Tg(Gnrhr-luc/EGFP)D8Mrln

Allele Symbol: Tg(Gnrhr-luc/EGFP)D8Mrln

Allele Name	transgene insertion D8, Glenn Merlino
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	GH; Glowing Head; rGH-ffLuc-eGFP
Gene Symbol and Name	Tg(Gnrhr-luc/EGFP)D8Mrln, transgene insertion D8, Glenn Merlino
Gene Synonym(s)
Promoter	Gnrhr, gonadotropin releasing hormone receptor, rat
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	luc, luciferase, firefly
Site of Expression	The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes.
Strain of Origin	C57BL/6
Chromosome	UN
Molecular Note	The transgenic construct contains a firefly luciferase (ffLuc)-eGFP fusion gene under the control of the rat growth hormone gene promoter and human growth hormone sequences. Reporter activity (by bioluminescent imaging) is detected in the anterior pituitary gland and testes.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Fluorescent Proteins
- Cancer Research
  - xenograft/transplant host
Cancer Research
- Toxicology
  - xenograft/transplant host

Mammalian Phenotype Terms by Genotype

References

Day CP; Carter J; Weaver Ohler Z; Bonomi C; El Meskini R; Martin P; Graff-Cherry C; Feigenbaum L; Tuting T; Van Dyke T; Hollingshead M; Merlino G. 2014. "Glowing head" mice: a genetic tool enabling reliable preclinical image-based evaluation of cancers in immunocompetent allografts. PLoS One 9(11):e109956PubMed: 25369133 MGI: J:221565

Additional - Tyr^c-Brd related

Additional - Tg(Gnrhr-luc/EGFP)D8Mrln related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Pyrosequencing:Tyr
- Probe:Fluorescent Proteins (Generic GFP)
- Standard PCR:Tg(Gnrhr-luc/EGFP)D8Mrl
- Sanger sequencing:Tyr-SEQ
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the Glowing Head on B6 mouse strain in a publication, please cite the originating article(s) and include JAX stock #027662 in your Materials and Methods section.

Strain Interest Registration

Please fill out the form below to indicate your interest in purchasing this JAX®Mice strain.
This information helps us manage the colony build and better meet the broad needs of the research community.
Please send any technical questions to Technical Support.

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Terms Of Use

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Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Glowing Head on B6

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tyrc-Brd

Allele Symbol: Tyrc-Brd

Tg(Gnrhr-luc/EGFP)D8Mrln

Allele Symbol: Tg(Gnrhr-luc/EGFP)D8Mrln

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tyrc-Brd related

Additional - Tg(Gnrhr-luc/EGFP)D8Mrln related

Genotyping Protocols

Breeding Considerations

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Tyr^c-Brd

Allele Symbol: Tyr^c-Brd

Additional - Tyr^c-Brd related