These Sox10-iCreERT2 mutant mice, carrying a transgene with tamoxifen-inducible optimized (improved) Cre recombinase under the control of the mouse Sox10 (SRY (sex determining region Y)-box 10) promoter, expresses iCreERT2 in oligodendrocyte lineage cells.
William Richardson, University College London
Genetic Background | Generation |
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?+pN1F6
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Starting at:
$278.00 Domestic price for female |
356.51 Domestic price for breeder pair |
The transcription factor encoded by the Sox10 gene is important in neural crest development, melanocyte development and homeostasis, myelination and mammary epithelial cell development. These Sox10-iCreERT2 mutant mice carry a transgene with tamoxifen-inducible optimized (improved) Cre recombinase under the control of the mouse Sox10 (SRY (sex determining region Y)-box 10) promoter. iCre is an "improved Cre", optimized with mammalian codon usage, removed putative cryptic splice sites, an altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, iCre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in oligodendrocyte lineage cells. When crossed to a ROSA-YFP reporter strain, the resulting double mutant mice, treated with tamoxifen, exhibited YFP expression in more than 90% of Sox10 expressing cells in the spinal cord, and 65% of Sox10 expressing cells in the cerebral cortex and corpus callosum one week after treatment. Homozygotes are viable and fertile. The Donating Investigator reports that there is no difference in phenotype between homozygotes and hemizygotes.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17ß-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
The 127 kb mouse phage artificial chromosome (PAC) RP21-427F18 containing the entire Sox10 gene, was modified by replacing the Sox10 open reading frame with an iCreERT2 fusion gene (an optimized variant of Cre recombinase fused to Cre-ERT2). DNA from the modified PAC clone was used as the source for the transgene, which was microinjected into the pronucleus of CBA;B6 fertilized eggs. No other genes on the RP21-427F18 PAC were modified or deleted. Founder line 388 was subsequently established. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | iCreERT2 is expressed in oligodendrocyte lineage cells. |
Allele Name | transgene insertion 388, William D Richardson |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Sox10-iCreERT2; Tg(Sox10-icre/ERT2)#aWdr |
Gene Symbol and Name | Tg(Sox10-icre/ERT2)388Wdr, transgene insertion 388, William D Richardson |
Gene Synonym(s) | |
Promoter | Sox10, SRY (sex determining region Y)-box 10, mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | iCreERT2 is expressed in oligodendrocyte lineage cells. |
Strain of Origin | C57BL/6 or CBA |
Chromosome | UN |
Molecular Note | The transgene was generated using the PAC RP21-427F18. Oligodendrocyte-specific expression of the inducible improved cre recombinase (icre/ERT2) was achieved by replacing the coding sequence of Sox10 with the fusion. Four founders were obtained and one (388) was selected for further study. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Sox10-iCreERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #027651 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Sox10-icre/ERT2)388Wdr |
Frozen Mouse Embryo | CBA;B6-Tg(Sox10-icre/ERT2)388Wdr/J | $2595.00 |
Frozen Mouse Embryo | CBA;B6-Tg(Sox10-icre/ERT2)388Wdr/J | $2595.00 |
Frozen Mouse Embryo | CBA;B6-Tg(Sox10-icre/ERT2)388Wdr/J | $3373.50 |
Frozen Mouse Embryo | CBA;B6-Tg(Sox10-icre/ERT2)388Wdr/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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