These wt h-parkin transgenic mice express human parkin (PARK2) in almost all ventral midbrain dopaminergic neurons (at a 7- to 8-fold increase compared to endogenous mouse parkin protein) and may be suitable for use in studies related to neurodegeneration and Parkinson Disease.
Konstanze F. Winklhofer, Ruhr University Bochum
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express the human (PARK2) under the direction of the mouse prion protein promoter.
Transgenic human parkin is expressed in almost all ventral midbrain dopaminergic neurons at a 7- to 8-fold increase and in striatal dopaminergic neurons at a 3- to 4-fold increase, compared to endogenous mouse parkin protein expression levels. When crossed to mice deficient in RET (ret proto-oncogene) the loss of dopaminergic neurons of the substantia nigra pars compacta is reduced, and total striatal dopamine levels are almost unaltered. Mice that are hemizygous for the transgene are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.
A transgenic construct containing 1398 bp of the human parkin ORF (PARK2) under control of the mouse Prnp was injected into the pronuclei of single cell FVB/NJ embryos. Founder line 196 was subsequently established. The mice were then backcrossed to C57BL/6 for 3 generations, and sperm was cryopreserved. Upon arrival to establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | PARK2, parkin RBR E3 ubiquitin protein ligase, chicken |
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Site of Expression |
Allele Name | transgene insertion 196, Konstanze Winklhofer |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | h-parkin |
Gene Symbol and Name | Tg(Prnp-PARK2)196Kfw, transgene insertion 196, Konstanze Winklhofer |
Gene Synonym(s) | |
Promoter | Prnp, prion protein, mouse, laboratory |
Expressed Gene | PARK2, parkin RBR E3 ubiquitin protein ligase, chicken |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | The transgenic construct contains 1398 bp of the human parkin ORF (PARK2) under control of the mouse prion promoter (Prnp). |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
When using the wt h-parkin mouse strain in a publication, please cite the originating article(s) and include JAX stock #027630 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Prnp-PARK2)196Kfw/Ekra |
Frozen Mouse Embryo | B6;FVB-Tg(Prnp-PARK2)196Kfw/EkraJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Prnp-PARK2)196Kfw/EkraJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Prnp-PARK2)196Kfw/EkraJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Tg(Prnp-PARK2)196Kfw/EkraJ Frozen Embryo | $3373.50 |
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