B6.129S6(C)-Zbtb46^tm1.1Kmm/J

Stock number: 027618
Product name: Zbtb46^gfp
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Zbtb46^gfp knock-in mice express EGFP in classical dendritic cells and committed progenitors.

Detailed description

An enhanced green fluorescent protein replaces exon 2, which encodes the start codon and the BTB domain of the zinc finger and BTB domain containing 46 (Zbtb46) gene. Zbtb46 is a transcription factor expressed in classical dendritic cells (cDCs) and their progenitors, where it regulates but is not required for differentiation. Homozygous Zbtb46^gfp mice are viable and fertile. GFP expression can be observed in cDCs, and committed erythroid progenitors and endothelial cells in lymphoid tissues. The absence of ZBTB46 in these cDCs represses alternate myeloid potential by maintaining granulocyte colony stimulating factor and leukemia inhibitory factor receptor expression.

Of note, this allele is also available on a 129S background (Stock No. 028823).

Development

A targeting vector was designed to replace exon 2 of the zinc finger and BTB domain containing 46 gene (Zbtb46) with an enhanced green fluorescent protein sequence, an SV40 polyadenylation signal, and a loxP-flanked PGK-neo^r cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ22 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric male from clone 102 was bred to 129SvEv females. Resulting progeny were bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054) to remove the neo cassette, and offspring were crossed to remove the cre-expressing transgene. Subsequently, these Zbtb46^gfp mice were bred to C57BL/6J mice for at least 9 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Zbtb46^tm1.1Kmm
Name: targeted mutation 1.1, Kenneth Murphy
MGI accession ID: MGI:5636376
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Zbtb46
Marker name: zinc finger and BTB domain containing 46
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in classical dendritic cells and committed erythroid progenitors, and in endothelial cells in lymphoid tissues.
Molecular note: A targeting vector was designed to replace exon 2, which encodes the start codon and the BTB domain of the zinc finger and BTB domain, with an enhanced green fluorescent protein followed by a polyadenylation signal and a floxed neo cassette. Microarray analysis confirmed the absence of transcript expression in dendritic cells. Cre-mediated recombination removed the floxed neo cassette.