Zbtb46gfp knock-in mice express EGFP in classical dendritic cells and committed progenitors.
Dr. Kenneth Murphy, Washington University School of Medicine
Genetic Background | Generation |
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N9+pN2F3
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Zbtb46 | zinc finger and BTB domain containing 46 |
An enhanced green fluorescent protein replaces exon 2, which encodes the start codon and the BTB domain of the zinc finger and BTB domain containing 46 (Zbtb46) gene. Zbtb46 is a transcription factor expressed in classical dendritic cells (cDCs) and their progenitors, where it regulates but is not required for differentiation. Homozygous Zbtb46gfp mice are viable and fertile. GFP expression can be observed in cDCs, and committed erythroid progenitors and endothelial cells in lymphoid tissues. The absence of ZBTB46 in these cDCs represses alternate myeloid potential by maintaining granulocyte colony stimulating factor and leukemia inhibitory factor receptor expression.
Of note, this allele is also available on a 129S background (Stock No. 028823).
A targeting vector was designed to replace exon 2 of the zinc finger and BTB domain containing 46 gene (Zbtb46) with an enhanced green fluorescent protein sequence, an SV40 polyadenylation signal, and a loxP-flanked PGK-neor cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ22 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric male from clone 102 was bred to 129SvEv females. Resulting progeny were bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054) to remove the neo cassette, and offspring were crossed to remove the cre-expressing transgene. Subsequently, these Zbtb46gfp mice were bred to C57BL/6J mice for at least 9 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in classical dendritic cells and committed erythroid progenitors, and in endothelial cells in lymphoid tissues. |
Allele Name | targeted mutation 1.1, Kenneth Murphy |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Zbtb46GFP |
Gene Symbol and Name | Zbtb46, zinc finger and BTB domain containing 46 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in classical dendritic cells and committed erythroid progenitors, and in endothelial cells in lymphoid tissues. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A targeting vector was designed to replace exon 2, which encodes the start codon and the BTB domain of the zinc finger and BTB domain, with an enhanced green fluorescent protein followed by a polyadenylation signal and a floxed neo cassette. Microarray analysis confirmed the absence of transcript expression in dendritic cells. Cre-mediated recombination removed the floxed neo cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Zbtb46gfp mouse strain in a publication, please cite the originating article(s) and include JAX stock #027618 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or wildtype for Zbtb46<tm1.1Kmm> |
Frozen Mouse Embryo | B6.129S6(C)-Zbtb46<tm1.1Kmm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6(C)-Zbtb46<tm1.1Kmm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6(C)-Zbtb46<tm1.1Kmm>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6(C)-Zbtb46<tm1.1Kmm>/J | $3373.50 |
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