Tph2f/f floxed mice may be useful for studying the role of serotonin in the maintenance of serotonin (5-HT) neurons and their effect on psychiatric and developmental disorders.
Zhou-Feng Chen, Washington University School of Medicine
Tph2f/f floxed mice possess loxP sites flanking exon 5 of the tryptophan hydroxylase 2 (Tph2) gene. TPH2 is an enzyme essential for the synthesis serotonin in the brain, which is required for neurogenesis, differentiation, and survival of central serotonin (5-HT) neurons. Serotonin is involved in numerous central nervous activities including the regulation of mood, appetite, sleep, memory and learning. Compromised serotonin neuronal phenotype may contribute to abnormal physiologic function and the onset of psychiatric disorders such as depression, anxiety and schizophrenia. In peripheral tissues, serotonin has a role in the maintenance of vascular tone and gut motility. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 5 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 5, with a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 5 of the tryptophan hydroxylase 2 (Tph2) gene. The construct was electroporated into 129S7/SvEvBrd-Hprt+-derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with wildtype females. The resulting Tph2f/f mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Zhou-Feng Chen|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Allele Synonym(s)||targeted mutation 1, Zhou-Feng Chen; Tph2tm1Zfc|
|Gene Symbol and Name||Tph2, tryptophan hydroxylase 2|
|Gene Synonym(s)||ADHD7; NTPH; expressed sequence AU043594; AU043594; Ntph|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 5.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Tph2f mouse strain in a publication, please cite the originating article(s) and include JAX stock #027590 in your Materials and Methods section.
|Heterozygous for Tph2<tm1Zfc>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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