EF1aCrb3-GFP KI mice may be useful as a Cre mediated reporter for visualizing membrane and/or tissue dynamics in living tissues.
Thomas J. Carroll, The University of Texas Southwestern Medical Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Eef1a1 | eukaryotic translation elongation factor 1 alpha 1 |
EF1aCrb3-GFP KI mice contain a floxed STOP cassette and a crumbs family member 3 (Crb3)-enhanced green fluorescent protein (GFP) fusion protein (Crb3-GFP) in exon 1 of the eukaryotic translation elongation factor 1 alpha 1 (Eef1a1) gene, abolishing Eef1a1 expression. Crumbs3 is a transmembrane protein localized to the apical membranes of epithelial cells. Homozygous Crb3-GFP males are viable and fertile, while homozygous females are not viable. In the absence of Cre, reporter gene expression is prevented by the STOP sequence. After removal of the loxP-flanked STOP cassette via cre-mediated recombination, GFP labels apical membranes in the majority of epithelial cell types where Cre recombinase is expressed.
The Crb3-GFP construct was designed with a loxP-flanked neomycin resistance (neo) cassette followed by a crumbs family member 3 (Crb3)-enhanced green fluorescent protein (GFP) fusion protein. The floxed-neo acts as a transcriptional STOP cassette. The construct was targeted to exon 1 of the eukaryotic translation elongation factor 1 alpha 1(Eef1a1) gene and was electroporated into 129S6/SvEvTac-derived SM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6N. These mice were maintained on a mixed 129;C57BL/6N background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Crb3, crumbs family member 3, mouse, laboratory |
Site of Expression | When combined with a Cre-recombinase-expressing strain, a Crb3-GFP fusion protein is expressed in epithelial cells. |
Allele Name | targeted mutation 1, Thomas Carroll |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | EF1aCrb3-GFP |
Gene Symbol and Name | Eef1a1, eukaryotic translation elongation factor 1 alpha 1 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Crb3, crumbs family member 3, mouse, laboratory |
Site of Expression | When combined with a Cre-recombinase-expressing strain, a Crb3-GFP fusion protein is expressed in epithelial cells. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 9 |
Molecular Note | The construct is designed with a loxP-flanked neomycin resistance (neo) cassette followed by a crumbs family member 3 (crumbs3)-green fluorescent protein (GFP) fusion protein. The floxed-neo acts as a transcriptional STOP cassette. The construct was targeted to exon 1 of the gene. |
When maintaining a live colony, heterozygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6NJ mice (Stock No. 005304). Homozygous mice are not viable.
When using the EF1aCrb3-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #027588 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Eif1a<tm1Tcrl> |
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