EF1aCrb3-GFP KI mice contain a floxed STOP cassette and a crumbs family member 3 (Crb3)-enhanced green fluorescent protein (GFP) fusion protein (Crb3-GFP) in exon 1 of the eukaryotic translation elongation factor 1 alpha 1 (Eef1a1) gene, abolishing Eef1a1 expression. Crumbs3 is a transmembrane protein localized to the apical membranes of epithelial cells. Homozygous Crb3-GFP males are viable and fertile, while homozygous females are not viable. In the absence of Cre, reporter gene expression is prevented by the STOP sequence. After removal of the loxP-flanked STOP cassette via cre-mediated recombination, GFP labels apical membranes in the majority of epithelial cell types where Cre recombinase is expressed.

EF1aCrb3-GFP KI mice may be useful as a Cre mediated reporter for visualizing membrane and/or tissue dynamics in living tissues.

A minimum of two mice must be ordered. Additional mice will be charged on a per-mouse basis. Click the “Details” button to learn more.

Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.