NFATc4-/- KO mice lack the translation initiation site, the regulatory domain that controls calmodulin-dependent cytoplasmic-to-nuclear translocation, and part of the DNA-binding domain. These mice may be useful for studying the role of NFAT transcription complexes on axonal and vascular outgrowth.
Gerald R Crabtree, Stanford University Medical Center
NFATc4-/- KO mice have a neo cassette replacing exons 1-2 and part of exon 3 encoding the nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4 (Nfatc4) gene with a neomycin resistance (neo) cassette. NFATc4 is a cytoplasmic subunit of an NFAT transcription complex which requires Ca2+/calcineurin signaling for nuclear import. The targeted region of the gene encodes amino acids 1-413 and contains the translation start site, a regulatory domain that controls calmodulin-dependent cytoplasmic-to-nuclear translocation, and part of the DNA-binding domain. Deletion of these regions abolishes gene expression. Homozygous mice are viable and fertile. When combined with deletions of other NFATc subunits, mice exhibit vascular and nervous system developmental defects resulting in embryonic lethality. NFATc subunits may have overlapping functions.
A targeting vector was designed to replace exons 1-2 and part of exon 3 encoding the nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4 (Nfatc4) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These NFATc4-/- mice were maintained on a mixed genetic background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Gerald R Crabtree|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Nfatc4tm1Grc; targeted mutation 1, Gerald R Crabtree|
|Gene Symbol and Name||Nfatc4, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4|
|Gene Synonym(s)||NF-ATC4; NFAT3; AW546455; RIKEN cDNA 3110041H08 gene; 3110041H08Rik; NF-AT3; expressed sequence AW107667; expressed sequence AW546455; AW107667; 3110041H08Rik|
|Strain of Origin||129S/SvEv|
|Molecular Note||Exons 1-2 and part of exon 3 were replaced with neomycin resistance gene via homologous recombination. This region of the gene encodes amino acids 1-413 and contains the translation start site, a regulatory domain that controls calmodulin-dependent cytoplasmic-to-nuclear translocation, and part of the DNA-binding domain. Western blot and RNase protection assay of homozygous mutant embryos verified the absence of gene expression.|
When maintaining a live colony, homozygous mice may be bred together.
When using the NFATc4- mouse strain in a publication, please cite the originating article(s) and include JAX stock #027581 in your Materials and Methods section.
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