B6;129S-Serpina3n^tm1.1Lbrl/J

Stock number: 027511
Product name: Serpina3n^fl
Research areas: Neurobiology Research

Description

Serpina3n^fl/fl floxed mice may be useful for developing therapeutic approaches to relieve receptor-activated neuropathic pain.

Detailed description

Serpina3n^fl/fl floxed mice possess loxP sites flanking exon 2 of the serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n) gene. SerpinA3n is a serine protease inhibitor, expressed in peripheral sensory neurons, that can reduce neuropathic pain hypersensitivity. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. Serpina3n^-/- mice exhibit increased neuropathic mechanical allodynia after nerve injury.

Development

A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the serine (or cysteine) peptidase inhibitor, clade A, member 3N (Serpina3n) gene. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with B6.129S4-Gt(ROSA)26Sor^tm1(FLP1)Dym/RainJ transgenic mice (Stock No. 009086) to delete the neo cassette. Serpina3n^fl/fl mice were maintained on a mixed B6;129 background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Serpina3n^tm1.1Lbrl
Name: targeted mutation 1.1, Stephen Liberles
MGI accession ID: MGI:5635495
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Synonyms: Serpina3n^fl
Marker symbol: Serpina3n
Marker name: serine (or cysteine) peptidase inhibitor, clade A, member 3N
Marker synonyms: AACT; ACT; alpha-1 antiproteinase; antitrypsin; CPi-26; GIG24; GIG25; Spi2.2; Spi-2.2; Spi2/eb.4; Spi2-2; SPI3; Spin2c; Spin3
Chromosome: 12
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2. Flp-mediated recombination removed the selection cassette.