B6.FVB-Tg(Ckm-SLC2A4/EGFP)1Samc/J

Stock number: 027496
Product name: HA-GLUT4-GFP
Research areas: Diabetes and Obesity Research, Metabolism Research, Research Tools

Description

HA-GLUT4-GFP transgenic mice express an HA-tagged human GLUT4-EGFP fusion protein under the control of the muscle creatinine kinase promoter. This strain may be useful for visualization of GLUT4 trafficking in response to insulin stimulation.

Detailed description

HA-GLUT4-GFP transgenic mice express an HA-tagged human GLUT4-EGFP fusion protein (coding sequence for the human SLC2A4 gene) under the control of the muscle creatinine kinase promoter. Mice homozygous for the transgene are viable and fertile. Transgenic mice overexpress GLUT4 (glucose transporter 4) by approximately 2.2 fold in skeletal muscle. This strain may be useful for visualization of GLUT4 trafficking in response to insulin stimulation.

Development

The transgenic construct consists of a hemaglutinin (HA) epitope and the human SLC2A4 (GLUT4) COOH terminus fused to an intracellular EGFP tag under the control of a muscle creatinine kinase promoter, enhancer 1, untranslated exon 1, 3kb of intron 1 including the enhancer 2 region, and the first 16 bp of exon 2 and a poly(A) signal. The HA epitope tag is in the first extracellular loop that is exposed on the cell surface. The 9 kb construct was microinjected into FVB/N fertilized oocytes. Founder animals were bred to C57BL/6 and offspring were backcrossed to C57BL/6 for 10 generations. Upon arrival, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Ckm-SLC2A4/EGFP)1Samc
Name: transgene insertion 1, Samuel Cushman
MGI accession ID: MGI:5641334
Generation method: Transgenic
Attributes: Reporter; Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Ckm-SLC2A4/EGFP)1Samc
Marker name: transgene insertion 1, Samuel Cushman
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: SLC2A4,solute carrier family 2 member 4,human; GFP,Green Fluorescent Protein,; HA,influenza hemagglutinin,
Site of expression: HA-GLUT4-GFP is expressed in skeletal muscle.
Molecular note: The transgenic construct consists of a hemaglutinin (HA) epitope and the human SLC2A4 (GLUT4) COOH terminus fused to an intracellular EGFP tag under the control of a muscle creatinine kinase promoter, enhancer 1, untranslated exon 1, 3kb of intron 1 (including the enhancer 2 region), and the first 16 bp of exon 2 and a poly(A) signal. The HA epitope tag is in the first extracellular loop that is exposed on the cell surface. Western blot analysis confirmed expression in skeletal muscle (soleus, gastrocnemius, extensor digitorum longus, flexor digitorum breviis). Expression is 2.2 fold higher than the endogenous protein.