These mice carry a β-geo genetrap/knockout mutation located in intron 2 of the mouse Kdm5b (Jarid1b) gene. Unlike other knockouts of this gene, the majority of homozygotes from this line are viable beyond embryonic and neonatal stages.
William G. Kaelin, Dana Farber Cancer Institute
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Gene trapped (Reporter, Null/Knockout) | Kdm5b | lysine (K)-specific demethylase 5B |
Histone demethylase JARID1B (Kdm5b, lysine (K)-specific demethylase 5B) promotes luminal lineage transcription programs in the mammary gland and fine-tunes systemic estrogen levels. It is overexpressed in HER2+ breast cancer and many breast cancer cell lines, making it a potential target for treatment.
These mice carry a β-geo genetrap/knockout mutation located in intron 2 of the mouse Kdm5b gene that creates a premature termination of the protein before the AT-rich interactive domain (ARID). Western blot analysis using an antibody against the C terminus shows that full-length protein is undetectable. β-geo can be used effectively for lacZ staining of cells normally expressing JARID1B.
Unlike other knockouts of this gene, the majority of homozygotes from this line are viable beyond embryonic and neonatal stages. Some animals die between 3-6 months of age for undetermined reasons. Others can live up to 2 years. Mice exhibit decreased body weight, premature mortality, decreased female fertility, lower uterine weight, and delayed mammary gland development. Serum estrogen levels are decreased and reduced mammary epithelial cell proliferation in observed in early puberty.
Ex vivo cells derived from the mammary glands of homozygotes exhibit diminished expression of key regulators of mammary gland morphogenesis and luminal lineage specification along with increased expression of a mammary stem cell signature.
A gene trap vector containing a splice-acceptor sequence upstream of β-geo (a fusion of β-galactosidase and neomycin phosphotransferase II) was introduced to intron 2 of the gene. This mutation was created in 129P2/OlaHsd-derived E14TG2a.4 embryonic stem (ES) cells. Correctly-targeted ES cells were injected into C57BL/6 blastocysts, and chimeric mice were bred with C57BL/6 mice. These mice were backcrossed to C57BL/6 for at least 7 generations, then backcrossed to FVB for 10 generations by the donating laboratory.
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
---|---|
Site of Expression | lacZ can be detected in cells normally expressing Kdm5b in the mammary gland. |
Allele Name | gene trap RRO123, BayGenomics |
---|---|
Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | Jarid1b- |
Gene Symbol and Name | Kdm5b, lysine (K)-specific demethylase 5B |
Gene Synonym(s) | |
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Site of Expression | lacZ can be detected in cells normally expressing Kdm5b in the mammary gland. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 1 |
General Note | E14TG2a.4 embryonic stem cells |
Molecular Note | A gene trap vector containing a splice-acceptor sequence upstream of beta-geo (a fusion of beta-galactosidase and neomycin phosphotransferase II) was introduced to intron 2 of the gene, resulting in premature termination of the protein before the AT-rich interactive domain (ARID). Western blot analysis using an antibody against the C terminus shows that full-length protein is undetectable. |
Heterozygotes are viable and fertile. The majority of homozygotes from this line are viable beyond embryonic and neonatal stages. Homozygous females have decreased fertility. Homozygous males have a mild fertility defect.
When using the Jarid1b KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027473 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Kdm5b<Gt(RRO123)Byg> |
Frozen Mouse Embryo | FVB.129P2(B6)-Kdm5b<Gt(RRO123)Byg>/KaelJFrozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB.129P2(B6)-Kdm5b<Gt(RRO123)Byg>/KaelJFrozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB.129P2(B6)-Kdm5b<Gt(RRO123)Byg>/KaelJFrozen Embryos | $3373.50 |
Frozen Mouse Embryo | FVB.129P2(B6)-Kdm5b<Gt(RRO123)Byg>/KaelJFrozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.