Pvalb-tdTomato transgenic reporter mice express tdTomato in parvalbumin positive neurons, and may be suitable for use in studies of fast-spiking interneurons in neurodegenerative and psychiatric disease, as well as learning and memory.
Guoping Feng, Massachusetts Institute of Technology
Genetic Background | Generation |
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N4+pN3F6
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
These transgenic mice express the red fluorescent protein, tdTomato, under the direction of the mouse Pvalb gene promoter. Transgene expression is detected in Pvalb -expressing neurons of the brain: somatosensory cortex, motor cortex, entorhinal cortex, striatum, thalamic reticular nucleus, hippocampal CA3 and CA1, and cerebellum. The tdTomato expression pattern is similar to the endogenous Pvalb gene expression pattern in the brain. High levels of tdTomato expression is observed in many brain regions including the neocortex. The source of the Pvalb gene promoter was the BAC clone RP24-306A6 which contains additional 5' sequences than previously generated Pvalb promoters used in transgenic lines (see for example, Stock No. 012355).
The mouse bacterial artificial chromosome (BAC) RP24-306A6 containing the entire Pvalb gene, was modified by first by a BAC trimming method to remove the Ift27 gene coding region. The second BAC recombineering round inserted the tdTomato-WPRE-BGHpA (bovine growth hormone
polyadenylation signal)-FRT-NEO-FRT vector directly downstream of the initiation start codon. The FRT site flanked NEO was then removed by transient FLP recombinase expression. Correctly targeted BAC DNA was used as the source for the transgene, which was linearized to remove it from the vector and then microinjected into C57BL/6NTac fertilized oocytes. The resulting transgenic mice from founder line 15 were subsequently backcrossed to C57BL/6J for at least 6 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | tdTomato is expressed in parvalbumin positive neurons. |
Allele Name | transgene insertion 15, Guoping Feng |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Pvalb-tdTomato)15Gfng, transgene insertion 15, Guoping Feng |
Gene Synonym(s) | |
Promoter | Pvalb, parvalbumin, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | tdTomato is expressed in parvalbumin positive neurons. |
Strain of Origin | C57BL/6NTac |
Chromosome | UN |
Molecular Note | The mouse bacterial artificial chromosome (BAC) RP24-306A6 containing the entire Pvalb gene, was modified first by a BAC trimming method to remove the Ift27 gene coding region. The second BAC recombineering round inserted the tdTomato-WPRE-BGHpA (bovine growth hormonepolyadenylation signal)-FRT-NEO-FRT vector directly downstream of the Pvalb initiation start codon. The FRT site flanked NEO was then removed by transient FLP recombinase expression. Correctly targeted BAC DNA was used as the source for the transgene, which was linearized to remove it from the vector. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
When using the Parvalbumin-TdTomato mouse strain in a publication, please cite the originating article(s) and include JAX stock #027395 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Pvalb-tdTomato)15Gfng |
Frozen Mouse Embryo | C57BL/6-Tg(Pvalb-tdTomato)15Gfng/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Pvalb-tdTomato)15Gfng/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Pvalb-tdTomato)15Gfng/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Pvalb-tdTomato)15Gfng/J | $3373.50 |
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