C57BL/6-Tg(Pvalb-tdTomato)15Gfng/J

Stock number: 027395
Product name: Parvalbumin-TdTomato
Research areas: Neurobiology Research, Research Tools

Description

Pvalb-tdTomato transgenic reporter mice express tdTomato in parvalbumin positive neurons, and may be suitable for use in studies of fast-spiking interneurons in neurodegenerative and psychiatric disease, as well as learning and memory.

Detailed description

These transgenic mice express the red fluorescent protein, tdTomato, under the direction of the mouse Pvalb gene promoter. Transgene expression is detected in Pvalb-expressing neurons of the brain: somatosensory cortex, motor cortex, entorhinal cortex, striatum, thalamic reticular nucleus, hippocampal CA3 and CA1, and cerebellum. The tdTomato expression pattern is similar to the endogenous Pvalb gene expression pattern in the brain. High levels of tdTomato expression is observed in many brain regions including the neocortex. The source of the Pvalb gene promoter was the BAC clone RP24-306A6 which contains additional 5' sequences than previously generated Pvalb promoters used in transgenic lines (see for example, Stock No. 012355).

Of note, parvalbumin may be expressed in sperm. Use of male Pvalb-tdTomato mice may result in germline tdTomato expression. If tdTomato expression in the male germline is a concern, researchers may consider breeding Pvalb-tdTomato females to non-transgenic males when creating mice for their experiments.

Development

The mouse bacterial artificial chromosome (BAC) RP24-306A6 containing the entire Pvalb gene, was modified by first by a BAC trimming method to remove the Ift27 gene coding region. The second BAC recombineering round inserted the tdTomato-WPRE-BGHpA (bovine growth hormone polyadenylation signal)-FRT-NEO-FRT vector directly downstream of the initiation start codon. The FRT site flanked NEO was then removed by transient FLP recombinase expression. Correctly targeted BAC DNA was used as the source for the transgene, which was linearized to remove it from the vector and then microinjected into C57BL/6NTac fertilized oocytes. The resulting transgenic mice from founder line 15 were subsequently backcrossed to C57BL/6J for at least 6 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Pvalb-tdTomato)15Gfng
Name: transgene insertion 15, Guoping Feng
MGI accession ID: MGI:5629292
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Pvalb-tdTomato)15Gfng
Marker name: transgene insertion 15, Guoping Feng
Chromosome: UN
Strain of origin: C57BL/6NTac
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: tdTomato is expressed in parvalbumin positive neurons of the brain: somatosensory cortex, motor cortex, entorhinal cortex, striatum, thalamic reticular nucleus, hippocampal CA3 and CA1, and cerebellum. High levels of tdTomato expression is observed in many brain regions including the neocortex.
Molecular note: The mouse bacterial artificial chromosome (BAC) RP24-306A6 containing the entire Pvalb gene, was modified first by a BAC trimming method to remove the Ift27 gene coding region. The second BAC recombineering round inserted the tdTomato-WPRE-BGHpA (bovine growth hormone polyadenylation signal)-FRT-NEO-FRT vector directly downstream of the Pvalb initiation start codon. The FRT site flanked NEO was then removed by transient FLP recombinase expression. Correctly targeted BAC DNA was used as the source for the transgene, which was linearized to remove it from the vector.