p47phox-/- mice contain a neo cassette interrupting exon 7, at amino acid 221, of the neutrophil cytosolic factor 1 (Ncf1) gene, abolishing gene function. Mice homozygous for this allele are viable and fertile. p47phox is the cytosolic subunit of phagocyte nicotinamide dinucleotide phosphate (NADPH) oxidase, and is required for its activation and subsequent production of superoxide. Superoxide is a toxic oxygen species that has been noted for the oxidative damage it causes during aging and in the development of some autoimmune diseases and cancers. Superoxide is also deployed by the immune system for use in oxygen-dependent killing mechanisms of invading pathogens. Mutations in p47phox has been linked to the onset of the immunodeficiency syndrome chronic granulomatous disease (CGD) which is characterized by extreme susceptibility to infection. Homozygous p47phox-/- mice are susceptible to infections by pathogens such as Staphylococcus spp., Lactobacillus, the Burkholderia cepacia complex, Serratia marcescens, Aspergillus spp., and Nocardia spp. but not Escherichia coli. Following intraperitoneal injection with thiogllycolate, mice exhibit inflammation recruiting twice as many leukocytes as controls. Homozygotes develop lethal infections and granulomatous inflammation similar to those encountered in human CGD patients. Leukocyte numbers and survival in heterozygotes are not significantly different from that seen in wildtype control mice. &nbsp;

p47phox-/- mice may be useful for studying role of NADPH oxidase in the prevention of and susceptibility to infections.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

<a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> for more information.