Overview

This miR-17~92^Δ19 knock-out strain is useful in studies related to the Mir19a and Mir19b-1 seed family, the Mirc1 microRNA cluster, and Myc-driven tumorigenesis and tumor progression.

Donating Investigator

Andrea Ventura, Memorial Sloan Kettering Cancer Center

Genetic overview

Genetic Background	Generation

Mirc1^tm2.1Aven

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Mirc1	microRNA cluster 1, including Mir17 through Mir92-1

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Research Applications

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The polycistronic Mirc1, microRNA cluster, or miR-17~92, consists of 6 members: Mir17, microRNA 17; Mir18, microRNA 18; Mir19a, microRNA 19a; Mir20a, microRNA 20a; Mir19b-1, microRNA 19b-1; and Mir92-1, microRNA 92-1. The region of nucleotides 2–7 from the mature miRNA 5´-end is called the miRNA seed sequence, which is essential for target recognition and binding to mRNA. The same seed sequence is often shared amongst miRNAs within the same family.

These miR-17~92^Δ19 mice have the miR-19 single seed family (Mir19a and Mir19b-1) knocked out of the Mirc1, microRNA cluster.

No Mir19a or Mir19b-1 miRNAs are detected by RT-qPCR and small RNA sequencing analysis of tail tissue from homozygotes. Mice that are homozygous for the targeted mutation are viable and fertile on the mixed B6;129 background, and slightly smaller than wildtype controls. Mendelian frequency for homozygotes on the C57BL/6J background is lower than expected (5%). 20% of homozygotes on the C57BL/6J background (N7 on the C57BL/6 background) exhibit vertebral fusion. The severity of the phenotype (including perinatal lethality) in homozygotes increases when the mice are backcrossed to C57BL/6.

This strain is part of a miR-17~92 (Mirc1, microRNA cluster 1, including Mir17 through Mir92-1) allelic series:
(Stock No. 027317), (Stock No. 027318),
(Stock No. 027319), (Stock No. 027320), (Stock No. 027321), (Stock No. 027322).

Development

A targeting vector containing a FRT site flanked neo selection cassette was utilized in the construction of this mutant. PCR-mediated mutagenesis was employed to delete
pre-miRNA sequences for Mir19a and Mir19b-1 from the targeting vector.

The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. Heterozygotes were then bred to beta-Actin-Flpe mice, carrying the Tg(ACTFLPe)9205Dym transgene, on the C57BL/6 background, to excise the FRT flanked NEO cassette. The mice were then backcrossed to C57BL/6J for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Han YC; Vidigal JA; Mu P; Yao E; Singh I; Gonzalez AJ; Concepcion CP; Bonetti C; Ogrodowski P; Carver B; Selleri L; Betel D; Leslie C; Ventura A. 2015. An allelic series of miR-17 approximately 92-mutant mice uncovers functional specialization and cooperation among members of a microRNA polycistron. Nat Genet 47(7):766-75PubMed: 26029871 MGI: J:223213

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Genetics

Mirc1^tm2.1Aven

Allele Symbol: Mirc1^tm2.1Aven

Allele Name	targeted mutation 2.1, Andrea Ventura
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	targeted mutation 2.1, Andrea Ventura; Mirc1^tm2.1Aven
Gene Symbol and Name	Mirc1, microRNA cluster 1, including Mir17 through Mir92-1
Gene Synonym(s)	Mir17-92; microRNA cluster 17-92; Mirn17-92; Mir17-92; Mirn17-92; miR-17-92; miR-17~92
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	14
Molecular Note	The Mir19a and Mir19b-1 genes were replaced by an FRT-flanked neomycin cassette. Flp-mediated recombination removed the FRT-flanked neo cassette.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Mirc1^tm2.1Aven/Mirc1^tm2.1Aven
involves: 129S4/SvJae * C57BL/6 * SJL/J

hematopoietic system phenotype

decreased B cell number
- decrease in total splenic B cells

decreased myeloid cell number
- decrease in total splenic myeloid cell (CD11b+) number

immune system phenotype

decreased B cell number
- decrease in total splenic B cells

The following phenotype relates to a compound genotype created using this strain

Genotype: Mirc1^tm2.1Aven/Mirc1^tm2.1Aven
B6.Cg-Mirc1

mortality/aging

perinatal lethality, incomplete penetrance
- heterozygote x heterozygote matings produce 5% homozygotes rather than the expected 25%

skeleton phenotype

vertebral fusion
- 20% of mice exhibit vertebral fusion

References

Han YC; Vidigal JA; Mu P; Yao E; Singh I; Gonzalez AJ; Concepcion CP; Bonetti C; Ogrodowski P; Carver B; Selleri L; Betel D; Leslie C; Ventura A. 2015. An allelic series of miR-17 approximately 92-mutant mice uncovers functional specialization and cooperation among members of a microRNA polycistron. Nat Genet 47(7):766-75PubMed: 26029871 MGI: J:223213

Additional - Mirc1^tm2.1Aven related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Mirc1^tm2.1Aven
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The severity of the phenotype (including perinatal lethality) in homozygotes increases when the mice are backcrossed to C57BL/6.
- Additional Breeding and Husbandry Support
Citation
When using the B6.Cg-Mirc1^tm2.1Aven/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #027319 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Mirc1<tm2.1Aven>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Mirc1tm2.1Aven

Allele Symbol: Mirc1tm2.1Aven

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Mirc1tm2.1Aven/Mirc1tm2.1Aveninvolves: 129S4/SvJae * C57BL/6 * SJL/J

hematopoietic system phenotype

immune system phenotype

Genotype: Mirc1tm2.1Aven/Mirc1tm2.1AvenB6.Cg-Mirc1

mortality/aging

skeleton phenotype

References

Additional - Mirc1tm2.1Aven related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Mirc1^tm2.1Aven

Allele Symbol: Mirc1^tm2.1Aven

Genotype: Mirc1^tm2.1Aven/Mirc1^tm2.1Aven
involves: 129S4/SvJae * C57BL/6 * SJL/J

Genotype: Mirc1^tm2.1Aven/Mirc1^tm2.1Aven
B6.Cg-Mirc1

Additional - Mirc1^tm2.1Aven related