STOCK Rspo3^tm1.1Jcob/J

Stock number: 027313
Product name: Rspo3^F
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 2 through 4 of the Rspo3 gene. This strain may be useful for generating conditional mutations in applications related to the activation of the Wnt/beta-catenin signaling pathway and regulation of development.

Detailed description

R-spondin is an activator of the Wnt/beta-catnenin signaling pathway and a ligand for LGR (leucine-rich G protein-coupled) receptors. These mice possess loxP sites on either side of exons 2 through 4 of the targeted Rspo3 gene, which encode the furin-like and TSR-1 domains. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in developing limb bud (see Stock No. 005584 for example), this mutant mouse strain may be useful in studies of limb development.

Development

A FRT site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a 5’ loxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into (129S6/SvEvTac x C57BL/6NCr)F1 derived G4 embryonic stem (ES) cells. Chimeric mice were generated by aggregating correctly targeted ES cells with CD-1 morula-stage embryos. The resulting male chimeric animals were crossed to CD-1 females. The mice were then crossed to transgenic mice (on the B6;SJL genetic background) expressing FLPe recombinase under the control of the human ACTB promoter (Stock No. 003800). Mice that retained the loxP site flanked exons 2-4 were then bred to remove the FLP recombinase transgene. Heterozygotes were crossed to generate homozygotes.

Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Rspo3^tm1.1Jcob
Name: targeted mutation 1.1, John Cobb
MGI accession ID: MGI:5443941
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rspo3
Marker name: R-spondin 3
Chromosome: 10
Strain of origin: (129S6/SvEvTac x C57BL/6NCrl)F1
Molecular note: A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed.