B6.129(Cg)-Snai3^tm1.1Jhws/J

Stock number: 027276
Product name: Snai3^FL
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Snai3^FL/FL floxed mice may be useful for studying the development and maintenance of immune tolerance.

Detailed description

Snai3^FL/FL floxed mice possess loxP sites flanking exon 1 of the snail family zinc finger 3 (Snai3) gene. SNAI3 is a transcriptional regulator that is highly expressed in double positive T cell lineages in skeletal muscle, cardiac muscle, and thymus, and in CD8+ T cells in the periphery. Expression is also seen in the kidney, liver and spleen. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues. Deletion of this gene in either T cell lineages or the entire animal had little effect upon animal development or T cell lineages/functions.

When bred to mice carrying one Snai2 KO allele from B6;129S1-Snai2^tm2Grid/J mice (Stock No. 010722) and subsequently bred to B6.Cg-Tg(Vav1-cre)A2Kio/J mice (Stock No. 008610) expressing Cre Recombinase in hematopoietic cells and their progenitors, and resulting cDKO mice exhibit severe impairment in both B and T cell generation. They also demonstrate an autoimmune phenotype manifested by the production of high levels of autoantibodies as early as 3 weeks of age and die by 30 days after birth. cDKO mice also display lymphopenia, alopecia, dermatitis, and runting.

Development

A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 1, and a second loxP site downstream of exon 1 of the snail family zinc finger 3 (Snai3) gene. The construct was electroporated into 129Sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred. Offspring were bred with Flp transgenic mice on a C57BL/6 background to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Resulting Snai3^FL/FL mice were bred to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Snai3^tm1.1Jhws
Name: targeted mutation 1.1, John Weis
MGI accession ID: MGI:5619843
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Snai3
Marker name: snail family zinc finger 3
Chromosome: 8
Strain of origin: 129/Sv
Molecular note: A targeting vector was designed to insert a loxP site followed by a FRT-flanked neomycin resistance (neo) cassette upstream of exon 1, and a second loxP site downstream of exon 1. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 1 floxed.