These Flnb knockout mice exhibit abnormal bone development with vertebral fusion and reduced ossification in long bones. This strain may be useful in studies of skeletal development and skeletal dysplasias such as spondylocarpaltarsal synostosis syndrome.
Volney L. Sheen, Beth Israel Deaconess Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Flnb | filamin, beta |
Filamin beta stabilizes 3D actin filament networks, connecting the cell membrane to the actin cytoskeleton. It has roles in regulating intracellular signaling, cell motility, organ development, tumor growth and metastasis. Mutations of FLNB are the cause of a group of human skeletal dysplasia disorders including spondylocarpotarsal syndrome, boomerang dysplasia, Larsen syndrome and atelosteogenesis I and III. These mice carry a knock out mutation for the Flnb gene in which exons 3 through 5 are replaced by a NEO cassette. Mice that are homozygous for the targeted mutation have a high death rate around the time of birth. Adult male homozygotes are fertile but exhibit mating problems due to deformity. Some surviving homozygous females exhibit dystocia. Heterozygotes are viable and fertile.
No gene product (protein) is detected by Western blot analysis of liver from 7 day old homozygotes and growth plate of the radius from E16.5 homozygotes. Surviving male homozygotes exhibit a small body size, lower body weight, shortened distal limbs, rib and vertebrae fusion, abnormal spinal curvatures, and dysmorphic facial/calvarial bones. Female homozygotes display a small body size, vertebral fusions and joint laxity. Homozygotes have a delay in endochondral bone development, reduced bone ossification, and disrupted extracellular matrix organization. Chondrocyte differentiation in long bones is delayed while proliferation is disrupted.
A targeting vector containing a NEO cassette was used to disrupt exons 3 through 5 of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived iTL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were tested for germline transmission.
The mice were then crossed to C57BL/6 and/or FVB.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Volney Sheen |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Flnb- |
Gene Symbol and Name | Flnb, filamin, beta |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 14 |
Molecular Note | Exons 3-5 were replaced by a neo selection cassette causing a frame shift mutation. Western blot analysis of P7 livers confirmed graded loss of protein expression in heterozygous and homozygous mutant mice. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Most homozygotes die perinatally. Surviving homozygous females exhibit dystocia and surviving homozygous males exhibit mating problems due to deformity.
When using the Flnb KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027233 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Flnb<tm1Vshn> |
Frozen Mouse Embryo | STOCK Flnb<tm1Vshn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Flnb<tm1Vshn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Flnb<tm1Vshn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Flnb<tm1Vshn>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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