B6.Cg-Tg(Cx3cl1*)1Jung/J

Stock number: 027119
Product name: Cx3cl1^105Δ
Research areas: Cell Biology Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

Cx3cl1^105Δ mice express a soluble form of CX3CL1 making these mice useful for studying leukocyte migration and trafficking, as well as integrin-independent adhesion and chemoattractive activity of monocytes.

Detailed description

Mice hemizygous for the BAC Cx3cl1^105Δ transgene are viable and fertile. The BAC has been modified to place a nonsense mutation at amino acid 105, in exon 3, of Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene followed by a myc-tag. CX3CL1 is the ligand for the chemokine receptor CX3CR1, and is involved in integrin-independent adhesion. CX3CL1 is expressed in mature neurons in the hippocampus, striatum, and cortical layer II and in epithelial cell layers. Expression is also seen in the lung in alveolar and bronchial epithelial cells, including Clara cells. In the kidney CX3CL1 is expressed in tubular epithelial cells and glomeruli, and in goblet cells and cells along the proximal and distal gastrointestinal tract. This mutation, in the mucin stalk domain, creates a truncated CX3CL1 protein lacking the mucin stalk, the transmembrane region, and the cytoplasmic tail. The mucin stalk functions to present the CX3C chemokine module, removal of which creates a secreted, truncated CX3CL1 protein. Cx3cl1^105Δ mice are useful when studying the differential chemoattractive activities of membrane tethered verses shed CX3CL1 proteins.

Development

Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (#RP24–147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to place a nonsense mutation at amino acid 105, in exon 3, followed by a myc-tag. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. This BAC was microinjected into fertilized CB6F1 oocytes. Founder Cx3cl1^105Δ mice were maintained by breeding with C57BL/6JOlaHsd inbred mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.

Allele

Symbol: Tg(Cx3cl1*)1Jung
Name: transgene insertion 1, Steffen Jung
MGI accession ID: MGI:5644036
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Cx3cl1*)1Jung
Marker name: transgene insertion 1, Steffen Jung
Chromosome: UN
Strain of origin: (BALB/c x C57BL/6)F1
Expressed genes: Cx3cl1,chemokine (C-X3-C motif) ligand 1,mouse, laboratory
Molecular note: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (#RP24147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to place a nonsense mutation at amino acid 105, in exon 3, followed by a myc-tag. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. The transgene encodes a secreted truncated protein lacking the mucin stalk.