Cx3cl1105Δ mice express a soluble form of CX3CL1 making these mice useful for studying leukocyte migration and trafficking, as well as integrin-independent adhesion and chemoattractive activity of monocytes.
Steffen Jung, Weizmann Institute of Science
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the BAC Cx3cl1105Δ transgene are viable and fertile. The BAC has been modified to place a nonsense mutation at amino acid 105, in exon 3, of Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene followed by a myc-tag. CX3CL1 is the ligand for the chemokine receptor CX3CR1, and is involved in integrin-independent adhesion. CX3CL1 is expressed in mature neurons in the hippocampus, striatum, and cortical layer II and in epithelial cell layers. Expression is also seen in the lung in alveolar and bronchial epithelial cells, including Clara cells. In the kidney CX3CL1 is expressed in tubular epithelial cells and glomeruli, and in goblet cells and cells along the proximal and distal gastrointestinal tract. This mutation, in the mucin stalk domain, creates a truncated CX3CL1 protein lacking the mucin stalk, the transmembrane region, and the cytoplasmic tail. The mucin stalk functions to present the CX3C chemokine module, removal of which creates a secreted, truncated CX3CL1 protein. Cx3cl1105Δ mice are useful when studying the differential chemoattractive activities of membrane tethered verses shed CX3CL1 proteins.
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (#RP24–147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to place a nonsense mutation at amino acid 105, in exon 3, followed by a myc-tag. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. This BAC was microinjected into fertilized CB6F1 oocytes. Founder Cx3cl1105Δ mice were maintained by breeding with C57BL/6JOlaHsd inbred mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | Cx3cl1, chemokine (C-X3-C motif) ligand 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Steffen Jung |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | CX3CL1105delta |
Gene Symbol and Name | Tg(Cx3cl1*)1Jung, transgene insertion 1, Steffen Jung |
Gene Synonym(s) | |
Promoter | Cx3cl1, chemokine (C-X3-C motif) ligand 1, mouse, laboratory |
Expressed Gene | Cx3cl1, chemokine (C-X3-C motif) ligand 1, mouse, laboratory |
Strain of Origin | (BALB/c x C57BL/6)F1 |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (#RP24147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to place a nonsense mutation at amino acid 105, in exon 3, followed by a myc-tag. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. The transgene encodes a secreted truncated protein lacking the mucin stalk. |
When maintaining a live colony hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J (Stock No. 000664). The donating investigator has not attempted to make this strain homozygous.
When using the Cx3cl1105Δ mouse strain in a publication, please cite the originating article(s) and include JAX stock #027119 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Cx3cl1*)1Jung |
Frozen Mouse Embryo | B6.Cg-Tg(Cx3cl1*)1Jung/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Cx3cl1*)1Jung/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Cx3cl1*)1Jung/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Cx3cl1*)1Jung/J | $3373.50 |
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