Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, 
the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mir15b/Mir16-2 (Mirc10) stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mirc10 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology. Additional information detailing the development of this mirKO mouse can be found <a href="http://rna.keck.ucsf.edu/node/96">here</a>).

These microRNA cluster 10 (or Mir15b/Mir16-2) conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. This mutant mouse strain may be useful in studies of microRNA biology.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.