NOD/ShiLtJ-H2-Ab1^em1Ygch/J

Stock number: 027057
Product name: NOD.H2-Ab-/- line 11
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These NOD mice contain a ZFN-mediated mutation that disrupts the MHC class II I-Ag⁷ molecule.

Detailed description

NOD.H2-Ab-/- line 11 mice contain a ZFN-mediated mutation targeted to exon 2 of the H2-Ab1 (histocompatibility 2, class II antigen A, beta 1) gene. The mutation disrupts the expression of the diabetes susceptible MHC class II I-Ag⁷ molecule uniquely expressed in NOD mice. I-Ag⁷ is involved in antigen presentation and T-cell activation, contributing to the development of Insulin-dependent diabetes mellitus (IDDM). NOD.H2-Ab-/- line 11 mice exhibit greatly reduced numbers of CD4 single positive T cells in the thymus and spleen.

Development

This zinc finger nuclease induced mutation targets exon 2 of the H2-Ab1 (histocompatibility 2, class II antigen A, beta 1) gene, creating a 17 bp deletion that disrupts normal expression of the MHC class II I-Ag⁷ molecule. The mRNA encoding the ZFN was injected into NOD/ShiLtJ (Stock No. 001976) one cell embryos and a mouse from founder line 11 was bred back to NOD/ShiLtJ. Resulting offspring were intercrossed. Upon arrival at The Jackson Laboratory, mice were bred to NOD/ShiLtJ for at least one generation to establish the colony.

Allele

Symbol: H2-Ab1^em1Ygch
Name: endonuclease-mediated mutation 1, Yi-Guang Chen
MGI accession ID: MGI:5618308
Generation method: Endonuclease-mediated
Attributes: Null/Knockout
Marker symbol: H2-Ab1
Marker name: histocompatibility 2, class II antigen A, beta 1
Chromosome: 17
Strain of origin: NOD/ShiLtJ
Molecular note: Zinc-finger nuclease technology generated a 17 bp deletion in exon 2 that disrupts normal expression of this diabetes susceptibility allele.