In this strain, a truncated form of human thioredoxin (TRX80) is expressed in macrophages and is combined with the Apoe KO (Apoetm1Unc) allele. These mice may be useful for studying Alzheimer’s disease and atherosclerosis.
Alan Remaley, NHLBI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Apoe | apolipoprotein E |
Allele Type |
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Transgenic (Inserted expressed sequence) |
TRX80 transgenic mice express a truncated form of the human thioredoxin TXN gene under the control of the human macrophage scavenger receptor (MSR1) enhancer/promoter. TXN is a dithiol reductant and antioxidant involved in the development of atherosclerosis and Alzheimer’s disease. The truncated form of TXN, TRX80, localizes to neurons and inhibits amyloid-beta aggregation. TRX80 levels are decreased in the brains of Alzheimer’s patients.
In this strain, the TRX80 transgene is expressed in macrogphages and is combined with the Apoe KO (Apoetm1Unc) allele. APOE expression is associated with amyloid beta deposition. This strain may be useful for studying Alzheimer’s disease and atherosclerosis.
The TRX80 transgene contains a truncated form of the human thioredoxin TXN gene under the control of the human macrophage scavenger receptor (MSR1) enhancer/promoter. TXN is truncated at the carboxy terminal from 105 residues to 80-84 amino acids. The construct was microinjected into C57BL/6N fertilized oocytes. The founder mouse 23 was mated to C57BL/6N. Progeny were then crossed to B6.129P2-Apoetm1Unc/J to develop a mice homozygous for the TRX80 transgene and the Apoetm1Unc allele. Upon arrival, mice were bred to B6.129P2-Apoetm1Unc/J (Stock 002052) for at least 1 generation to establish the colony.
Expressed Gene | TXN, thioredoxin, human |
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Site of Expression |
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | AopE(-); apoE-; APOE KO; Apoetm1Un; apoE0; ApoE-KO; EKO; epsilon-; mE-; mEKO |
Gene Symbol and Name | Apoe, apolipoprotein E |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. |
Mutations Made By | Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill |
Allele Name | transgene insertion 23, Alan Remaley |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | TRX80 |
Gene Symbol and Name | Tg(MSR1-TXN*)23Arem, transgene insertion 23, Alan Remaley |
Gene Synonym(s) | |
Promoter | MSR1, macrophage scavenger receptor 1, human |
Expressed Gene | TXN, thioredoxin, human |
Strain of Origin | C57BL/6N |
Chromosome | UN |
Molecular Note | The transgene construct contains a truncated form of the human thioredoxin TXN gene under the control of the human macrophage scavenger receptor (MSR1) enhancer/promoter. TXN is truncated at the carboxy terminal from 105 residues to 80-84 amino acids. |
While maintaining a live colony, these mice are bred as homozygous for both alleles.
When using the apoE-; TRX80 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37627 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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