B6.Cg-Apoe^tm1Unc Tg(MSR1-TXN*)23Arem/Mmjax

Stock number: 027052
Product name: apoE-; TRX80
Research areas: Cardiovascular Research, Mouse/Human Gene Homologs, Neurobiology Research

Description

In this strain, a truncated form of human thioredoxin (TRX80) is expressed in macrophages and is combined with the Apoe KO (Apoe^tm1Unc) allele. These mice may be useful for studying Alzheimer's disease and atherosclerosis.

Detailed description

TRX80 transgenic mice express a truncated form of the human thioredoxin TXN gene under the control of the human macrophage scavenger receptor (MSR1) enhancer/promoter. TXN is a dithiol reductant and antioxidant involved in the development of atherosclerosis and Alzheimer's disease. The truncated form of TXN, TRX80, localizes to neurons and inhibits amyloid-beta aggregation. TRX80 levels are decreased in the brains of Alzheimer's patients. In this strain, the TRX80 transgene is expressed in macrogphages and is combined with the Apoe KO (Apoe^tm1Unc) allele. APOE expression is associated with amyloid beta deposition. This strain may be useful for studying Alzheimer's disease and atherosclerosis.

Development

The TRX80 transgene contains a truncated form of the human thioredoxin TXN gene under the control of the human macrophage scavenger receptor (MSR1) enhancer/promoter. TXN is truncated at the carboxy terminal from 105 residues to 80-84 amino acids. The construct was microinjected into C57BL/6N fertilized oocytes. The founder mouse 23 was mated to C57BL/6N. Progeny were then crossed to B6.129P2-Apoe^tm1Unc/J to develop a mice homozygous for the TRX80 transgene and the Apoe^tm1Unc allele. Upon arrival, mice were bred to B6.129P2-Apoe^tm1Unc/J (Stock 002052) for at least 1 generation to establish the colony.

Allele

Symbol: Apoe^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857129
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Apoe
Marker name: apolipoprotein E
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Molecular note: Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE.

Allele

Symbol: Tg(MSR1-TXN*)23Arem
Name: transgene insertion 23, Alan Remaley
MGI accession ID: MGI:5613981
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(MSR1-TXN*)23Arem
Marker name: transgene insertion 23, Alan Remaley
Chromosome: UN
Strain of origin: C57BL/6N
Expressed genes: TXN,thioredoxin,human
Molecular note: The transgene construct contains a truncated form of the human thioredoxin TXN gene under the control of the human macrophage scavenger receptor (MSR1) enhancer/promoter. TXN is truncated at the carboxy terminal from 105 residues to 80-84 amino acids.