aroCre mice express Cre recombinase in portions of the amygdala. They may be useful in applications examining sexually dimorphic social behaviors.
Nirao Shah, Stanford University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Cyp19a1 | cytochrome P450, family 19, subfamily a, polypeptide 1 |
aroCre mice express Cre recombinase under the direction of the Cyp19a1 (cytochrome P450, family 19, subfamily a, polypeptide 1) gene. The presence of cre does not affect the expression of Cyp19a1. CYP19A1 encodes aromatase, an enzyme responsible for the aromatization of androgens into estrogens in the brain. Aromatase is expressed in the posterodorsal medial amygdala of the male brain where it controls neural circuits underlying courtship vocalization, mating, aggression, and territory marking in male mice. Brains of female mice contain lower levels of aromatase. Homozygous mice are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.
Injection of an adeno-associated virus (AAV) encoding Cre-dependent caspase-3 into mice carrying this aroCre and one copy of a aro- allele triggers apoptosis exclusively in aromatase+ cells. Males show no changes in courtship or mating habits, but do show increased aggression towards other males. Females display increased maternal aggression towards intruders.
When injected with cre-dependent DREADD-Gi:mCherry, which inhibits GABAergic neurons, mice show increased latency of attack on other males.
A targeting construct was designed to insert an internal ribosomal entry site (IRES) and a Cre Recombinase gene, followed by a frt-flanked neomycin resistance (neo) cassette into the 3’ UTR of the Cyp19a1 (cytochrome P450, family 19, subfamily a, polypeptide 1) gene. This construct was electroporated into 129/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6J females. Offspring were bred to B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice Stock No. 005703) and resulting progeny were crossed to remove the flp-expressing transgene. aroCre mice were backcrossed for at least 5 generations to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in portions of the amygdala. |
Allele Name | targeted mutation 2.1, Nirao Shah |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | aroCre; aromataseCre |
Gene Symbol and Name | Cyp19a1, cytochrome P450, family 19, subfamily a, polypeptide 1 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in portions of the amygdala. |
Strain of Origin | 129S/SvEv |
Chromosome | 9 |
Molecular Note | A targeting construct was designed to insert an internal ribosomal entry site (IRES) and a Cre recombinase gene, followed by a FRT-flanked neomycin resistance (neo) cassette downstream of the 3' UTR. Flp-mediated recombination removed the FRT-flanked neo cassette. |
When maintaining a live colony homozygous mice may be bred together.
When using the aroCre mouse strain in a publication, please cite the originating article(s) and include JAX stock #027038 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Cyp19a1<tm2.1(cre)Shah> |
Frozen Mouse Embryo | B6.129S(SJL)-Cyp19a1<tm2.1(cre)Shah>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S(SJL)-Cyp19a1<tm2.1(cre)Shah>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S(SJL)-Cyp19a1<tm2.1(cre)Shah>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S(SJL)-Cyp19a1<tm2.1(cre)Shah>/J | $3373.50 |
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